Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs.

Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs. Research Abstract Details 

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Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs. Abstract Text:

Campylobacter fetus cells possess multiple promoterless sap homologs, each capable of expressing a surface layer protein (SLP) by utilizing a unique promoter present on a 6.2-kb invertible element. Each sap homolog includes a 626-bp 5' conserved region (FCR) with 74 bp upstream and 552 bp within the open reading frame. After DNA inversion, the splice is seamless because the FCRs are identical. In mutant strain 23D:ACA2K101, in which sapA and sapA2 flanking the invertible element in opposite orientations were disrupted by promoterless chloramphenicol resistance (Cm(r)) and kanamycin resistance (Km(r)) cassettes, respectively, the frequency of DNA inversion is 100-fold lower than that of wild-type strain 23D. To define the roles of a 15-bp inverted repeat (IR) and a Chi-like site (CLS) in the FCR, we mutagenized each upstream of sapA2 in 23D:ACA2K101 by introducing NotI and KpnI sites to create strains 23D:ACA2K101N and 23D:ACA2K101K, respectively. Alternatively selecting colonies for Cm(r) or Km(r) showed that mutagenizing the IR or CLS had no apparent effect on the frequency of the DNA inversion. However, mapping the unique NotI or KpnI site in relation to the Cm(r) or Km(r) cassette in the cells that changed phenotype showed that splices occurred both upstream and downstream of the mutated sites. PCR and sequence analyses also showed that the splice could occur in the 425-bp portion of the FCR downstream of the cassettes. In total, these data indicate that C. fetus can use multiple sites within the FCR for its sap-related DNA inversion.

Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs. Publishing Authors By Initials

For similar genetic processes: recombination, genetic research abstracts see: genetic processes: recombination, genetic research

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Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 183

Page Numbers: 6654-61

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 24

MONTH: Nov

YEAR: 2001

Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs. Keywords Mesh Terms:

KEYWORDS: Recombination, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs. Information

Substance Name: S-layer proteins

Registry Number: 0

Grant and Affiliation Information for Campylobacter fetus uses multiple loci for DNA inversion within the 5' conserved regions of sap homologs.

AFFILIATION: Division of Infectious Diseases, Department of Medicine, New York University School of Medicine, New York, New York 10016, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R29 AI43548

ACRONYM: AI

MEDLINETA: J Bacteriol

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