Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes.

Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes. Research Abstract Details 

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Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes. Abstract Text:

S Murase,K Yamada,Y Naito,J Hirose,H Okuyama,

The lysophosphoinositide-specific phospholipase C (lysoPI-PLase C) in porcine platelet membranes had an optimal pH of 9.2 and the activity at a physiological pH of 7.3 was 20% of the maximum in the absence of added divalent metals (Murase, S. et al. (1985) J. Biol. Chem. 260, 262). The activity was completely inhibited by 1 mM EGTA in the assay mixture but was restored by addition of excess Ca2+ or Mn2+, indicating that this is a metalloenzyme. However, membranes pretreated with 1 mM EGTA and washed with buffer retained full activity at a free Ca2+ concentration of 5 nM and no stimulation was observed by added Ca2+ at pH 9.2. In contrast to the results obtained at pH 9.2, addition of Ca2+ stimulated lysoPI-PLase C activity severalfold at pH 7.3, apparently by shifting down the optimal pH and broadening the pH profile. The effect of Ca2+ at pH 7.3 was to enhance Vmax with no significant change in Km value. The stimulatory effect of Ca2+ at pH 7.3 alone did not appear to be of physiological significance since millimolar concentrations of Ca2+ were necessary to reach the maximum activity. However, a shift in pH had a profound effect on the Ca2+-dependency of the activity. A rise in 2 pH units increased the apparent affinity for Ca2+ 10,000-fold. These results indicate that the alkalinization and the rise in free Ca2+ concentration known to occur in stimulated platelets could synergistically provide conditions under which the lysoPI-PLase C exerts its activity when the substrate lysoPI is generated by phospholipase A.

Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes. Publishing Authors By Initials

S Murase,K Yamada,Y Naito,J Hirose,H Okuyama,

For similar type c phospholipases research abstracts see: type c phospholipases research

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Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 104

Page Numbers: 270-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1988

Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes. Keywords Mesh Terms:

KEYWORDS: Type C Phospholipases

MESH TERMS: blood

Chemical & Substance for Abstract: Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes. Information

Substance Name: Type C Phospholipases

Registry Number: EC 3.1.4.-

Grant and Affiliation Information for Calcium-requirement and its pH dependency of lysophosphoinositide-specific phospholipase C in porcine platelet membranes.

AFFILIATION: Faculty of Pharmaceutical Sciences, Nagoya City University, Aichi.

Country: JAPAN

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MEDLINETA: J Biochem

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