Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention.

Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. Research Abstract Details 

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Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. Abstract Text:

Tibor Kristian,Natalia B Pivovarova,Gary Fiskum,S Brian Andrews,

Both isolated brain mitochondria and mitochondria in intact neurons are capable of accumulating large amounts of calcium, which leads to formation in the matrix of calcium- and phosphorus-rich precipitates, the chemical composition of which is largely unknown. Here, we have used inhibitors of the mitochondrial permeability transition (MPT) to determine how the amount and rate of mitochondrial calcium uptake relate to mitochondrial morphology, precipitate composition, and precipitate retention. Using isolated rat brain (RBM) or liver mitochondria (RLM) Ca(2+)-loaded by continuous cation infusion, precipitate composition was measured in situ in parallel with Ca(2+) uptake and mitochondrial swelling. In RBM, the endogenous MPT inhibitors adenosine 5'-diphosphate (ADP) and adenosine 5'-triphosphate (ATP) increased mitochondrial Ca(2+) loading capacity and facilitated formation of precipitates. In the presence of ADP, the Ca/P ratio approached 1.5, while ATP or reduced infusion rates decreased this ratio towards 1.0, indicating that precipitate chemical form varies with the conditions of loading. In both RBM and RLM, the presence of cyclosporine A in addition to ADP increased the Ca(2+) capacity and precipitate Ca/P ratio. Following MPT and/or depolarization, the release of accumulated Ca(2+) is rapid but incomplete; significant residual calcium in the form of precipitates is retained in damaged mitochondria for prolonged periods.

Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. Publishing Authors By Initials

T Kristian,NB Pivovarova,G Fiskum,SB Andrews,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Journal of neurochemistry

VOLUME: 102

Page Numbers: 1346-56

Journal Abbreviation: J. Neurochem.

ISSN: 0022-3042

DAY: 3

MONTH: Aug

YEAR: 2007

Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. Information

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LANGUAGE: eng

NlmUniqueID: 2985190

Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention. Information

Substance Name: Phosphorus

Registry Number: 7723-14-0

Grant and Affiliation Information for Calcium-induced precipitate formation in brain mitochondria: composition, calcium capacity, and retention.

AFFILIATION: Department of Anesthesiology, University of Maryland, School of Medicine, Baltimore, Maryland 21201, USA. tkris001@umaryland.edu

Country: England

AGENCY: United States NINDS

GRANT: R21NS050653

ACRONYM: NS

MEDLINETA: J Neurochem

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