Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator.

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Abstract Text:

Intracellular Ca(2+) regulates numerous proteins and cellular functions and can vary substantially over submicron and submillisecond scales, so precisely localized fast detection is desirable. We have created a approximately 1-kDa biarsenical Ca(2+) indicator, called Calcium Green FlAsH (CaGF, 1), to probe [Ca(2+)] surrounding genetically targeted proteins. CaGF attached to a tetracysteine motif becomes ten-fold more fluorescent upon binding Ca(2+), with a K(d) of approximately 100 microM, <1-ms kinetics and good Mg(2+) rejection. In HeLa cells expressing tetracysteine-tagged connexin 43, CaGF labels gap junctions and reports Ca(2+) waves after injury. Total internal reflection microscopy of tetracysteine-tagged, CaGF-labeled alpha(1C) L-type calcium channels shows fast-rising depolarization-evoked Ca(2+) transients, whose lateral nonuniformity suggests that the probability of channel opening varies greatly over micron dimensions. With moderate Ca(2+) buffering, these transients decay surprisingly slowly, probably because most of the CaGF signal comes from closed channels feeling Ca(2+) from a tiny minority of clustered open channels. With high Ca(2+) buffering, CaGF signals decay as rapidly as the calcium currents, as expected for submicron Ca(2+) domains immediately surrounding active channels. Thus CaGF can report highly localized, rapid [Ca(2+)] dynamics.

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Publishing Authors By Initials

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

PUBMED ID PMID:

MEDLINE DATE:

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nature chemical biology

VOLUME: 3

Page Numbers: 423-31

Journal Abbreviation: Nat. Chem. Biol.

ISSN: 1552-4450

DAY: 17

MONTH: 06

YEAR: 2007

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101231976

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator.

AFFILIATION: Howard Hughes Medical Institute, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0647, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS27177

ACRONYM: NS

MEDLINETA: Nat Chem Biol

REFSOURCE: Nat Chem Biol. 2007 Jul;3(7):369-70

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator Related Publications

• A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure.
• Anti-Ca2+ channel antibody attenuates Ca2+ currents and mimics cerebellar ataxia in vivo.
• Aptamers switch on fluorescence of triphenylmethane dyes.
• Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator.
• Dentate gyrus-specific manipulation of beta-Ca2+/calmodulin-dependent kinase II disrupts memory consolidation.
• Evolution of new nonantibody proteins via iterative somatic hypermutation.
• Evolving proteins in mammalian cells using somatic hypermutation.
• Fluorescence ratio imaging of dynamic intracellular signals.
• From the Cover: A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals.
• Functional disturbances in the striatum by region-specific ablation of NMDA receptors.
• Improving the photostability of bright monomeric orange and red fluorescent proteins.
• Isoform-specific PKA dynamics revealed by dye-triggered aggregation and DAKAP1alpha-mediated localization in living cells.
• Maintenance of superior learning and memory function in NR2B transgenic mice during ageing.
• Measuring calcium signaling using genetically targetable fluorescent indicators.
• Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters.
• Preparation of the membrane-permeant biarsenicals FlAsH-EDT(2) and ReAsH-EDT(2) for fluorescent labeling of tetracysteine-tagged proteins.
• Quantum dots provide an optical signal specific to full collapse fusion of synaptic vesicles.
• Real-time neural coding of memory.
• Roger Tsien: bringing color to cell biology. A passion for chemistry, color, and conversations with cells is what drives Roger Tsien. Interview by Ruth Williams.
• Synapse-specific adaptations to inactivity in hippocampal circuits achieve homeostatic gain control while dampening network reverberation.
• The Timothy syndrome mutation differentially affects voltage- and calcium-dependent inactivation of CaV1.2 L-type calcium channels.
• The sequence of events that underlie quantal transmission at central glutamatergic synapses.
• [FETAL ERYTHROCYTES AND HEMOGLOBIN IN INFANTS AND CHILDREN--INFLUENCE OF EXCHANGE TRANSFUSION.]