Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans.

Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans. Research Abstract Details 

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Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans. Abstract Text:

Salivary histatin 5 (Hst 5) kills the fungal pathogen C. albicans via a mechanism that involves binding and subsequent efflux of cellular ATP. Our aims were to identify inorganic ions found in saliva that influence Hst 5 fungicidal activity. Increasing ionic strength with relevant salivary anions (Cl(-) and CO(3)(-)) did not reduce Hst 5 binding or uptake by yeast cells, but reduced the Hst-induced efflux of ATP. Extracellular MgCl(2) (25 mM) maximally inhibited 30-40% of Hst 5 killing with 40% reduction in ATP efflux, while pre-treatment of cells with only 2 mM CaCl(2) inhibited 80-90% of killing, and prevented ATP efflux. Loss of fungicidal activity by the addition of CaCl(2) or MgCl(2) was a result of inhibition of binding of Hst 5 to C. albicans cells. Calcium is a potent inhibitor of Hst 5 candidacidal activity at physiological concentrations and may be the primary salivary ion responsible for the masking effect of saliva.

Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans. Publishing Authors By Initials

For similar proteins: salivary proteins research abstracts see: proteins: salivary proteins research

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Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of dental research

VOLUME: 82

Page Numbers: 748-52

Journal Abbreviation: J. Dent. Res.

ISSN: 0022-0345

DAY: 18

MONTH: Sep

YEAR: 2003

Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 354343

Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans. Keywords Mesh Terms:

KEYWORDS: Salivary Proteins

MESH TERMS: antagonists & inhibitors

Chemical & Substance for Abstract: Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans. Information

Substance Name: Magnesium Chloride

Registry Number: 7786-30-3

Grant and Affiliation Information for Calcium blocks fungicidal activity of human salivary histatin 5 through disruption of binding with Candida albicans.

AFFILIATION: Department of Oral Biology, State University of New York, Buffalo, NY 14214, USA. edgerto@buffalo.edu

Country: United States

AGENCY: United States NIDCR

GRANT: R01 DE010641-09

ACRONYM: DE

MEDLINETA: J Dent Res

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