Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture.

Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture. Abstract Text:

M Akiyama,Y Minami,T Nakajima,T Moriya,S Shibata,

Mammalian circadian clock genes Per1 and Per2 are rhythmically expressed not only in the suprachiasmatic nucleus where the mammalian circadian clock exists, but also in other brain regions and peripheral tissues. The induced circadian oscillation of Per genes after treatment with high concentrations of serum or various drugs in cultured cells suggests the ubiquitous existence of the oscillatory mechanism. These treatments also result in a rapid surge of expression of Per1. It has been shown that multiple signaling pathways are involved in Per1 gene induction in culture cells. We used a dispersed primary cell culture made up of mouse cerebellar granule cells to examine the stimuli inducing the mPer genes and their signaling pathways in neuronal tissues expressing mPer genes. We demonstrated that mPer1, but not mPer2, mRNA expression was dependent on the depolarization state controlled by extracellular KCl concentration in the granule cell culture. Nifedipine treatment reduced mPer1 induction, suggesting that mPer1 mRNA expression depends on intracellular calcium concentration regulated through a voltage-dependent Ca2+ channel. Transient mPer1 mRNA induction was observed after elevating KCl concentration in the medium from 5 mM to 25 mM. This increased expression was suppressed by a calmodulin antagonist, or CaMKII/IV inhibitor, but not by MEK inhibitors. Addition of pituitary adenylate cyclase-activating polypeptide-38 to the medium also induced transient Per1 gene expression. This induction was mimicked by dibutyryl-cAMP and suppressed by a protein kinase A (PKA) inhibitor, but not by MEK inhibitors. These results suggest that Ca2+/calmodulin-dependent protein kinase II/IV- and PKA-dependent pathways are involved in high-KCl and PACAP-induced mPer1 induction, respectively, and neural tissues use multiple signaling pathways for mPer1 induction similar to culture cells.

Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture. Publishing Authors By Initials

M Akiyama,Y Minami,T Nakajima,T Moriya,S Shibata,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

PUBMED ID PMID:

MEDLINE DATE:

Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of neurochemistry

VOLUME: 78

Page Numbers: 499-508

Journal Abbreviation: J. Neurochem.

ISSN: 0022-3042

DAY: 19

MONTH: Aug

YEAR: 2001

Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985190

Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture. Information

Substance Name: Calcium-Calmodulin-Dependent Protein Kin

Registry Number: EC 2.7.11.17

Grant and Affiliation Information for Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture.

AFFILIATION: Department of Pharmacology and Brain Science, School of Human Sciences, Waseda University, Tokorozawa, Saitama, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Neurochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture Related Publications

• 2-Amino-5-(N-ethylcarboxamido)-pentanoic Acid from Green Tea Leaves.
• Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift.
• Calcium and pituitary adenylate cyclase-activating polypeptide induced expression of circadian clock gene mPer1 in the mouse cerebellar granule cell culture.
• Circadian profile of Per gene mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, and pineal body of aged rats.
• Confirmation of New, High-mass Saponins from Gleditsia japonica by Field Desorption Mass Spectrometry*.
• Constant light housing attenuates circadian rhythms of mPer2 mRNA and mPER2 protein expression in the suprachiasmatic nucleus of mice.
• Daily injection of insulin attenuated impairment of liver circadian clock oscillation in the streptozotocin-treated diabetic mouse.
• Differential daily expression of Per1 and Per2 mRNA in the suprachiasmatic nucleus of fetal and early postnatal mice.
• Differential subcellular localization of COX-2 in macrophages phagocytosing heat-killed Mycobacterium bovis BCG.
• Extended action of MKC-242, a selective 5-HT(1A) receptor agonist, on light-induced Per gene expression in the suprachiasmatic nucleus in mice.
• Gleditsia saponins.
• In vivo evaluation of oxygen consumption by arteriolar walls in skeletal muscle.
• Inhibitory action of brotizolam on circadian and light-induced per1 and per2 expression in the hamster suprachiasmatic nucleus.
• Lack of increases in methylation at three CpG-rich genomic loci in non-mitotic adult tissues during aging.
• MAP kinase-dependent induction of clock gene expression by alpha 1-adrenergic receptor activation.
• Methamphetamine-induced, suprachiasmatic nucleus-independent circadian rhythms of activity and mPer gene expression in the striatum of the mouse.
• Mode of interaction of two fluorinated-hydrogenated hybrid amphiphiles with dipalmitoylphosphatidylcholine (DPPC) at the air-water interface.
• Modulation of mPer1 gene expression by anxiolytic drugs in mouse cerebellum.
• Nonphotic entrainment by 5-HT1A/7 receptor agonists accompanied by reduced Per1 and Per2 mRNA levels in the suprachiasmatic nuclei.
• Physical and inflammatory stressors elevate circadian clock gene mPer1 mRNA levels in the paraventricular nucleus of the mouse.
• Pituitary adenylate cyclase-activating polypeptide produces a phase shift associated with induction of mPer expression in the mouse suprachiasmatic nucleus.
• Restricted feeding entrains liver clock without participation of the suprachiasmatic nucleus.
• Restricted-feeding-induced anticipatory activity rhythm is associated with a phase-shift of the expression of mPer1 and mPer2 mRNA in the cerebral cortex and hippocampus but not in the suprachiasmatic nucleus of mice.
• Sensitized increase of period gene expression in the mouse caudate/putamen caused by repeated injection of methamphetamine.
• Structures of linear furano- and simple-coumarin glycosides of bai-hua qian-hu1.
• Studies on Coumarins of a Chinese Drug "Qian-Hu" V. Coumarin-glycosides from "Zi-Hua Qian-Hu"1.
• Studies on coumarins of a chinese drug "qian-hu".
• Studies on coumarins of a chinese drug "qian-hu".
• The role of Clock in the plasticity of circadian entrainment.
• Toxaphene and other organochlorine compounds in pintails (Anas acuta) from Saitama Kamoba in Japan during Oct 2000-Feb 2002.