CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory.

CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. Research Abstract Details 

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CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. Abstract Text:

Yanjun Song,Feng He,Gengqiang Xie,Xiaoyan Guo,Yanjuan Xu,Yixu Chen,Xuehong Liang,Igor Stagljar,Dieter Egli,Jun Ma,Renjie Jiao,

DNA synthesis during S-phase and upon DNA repair is accompanied by chromatin assembly. The chromatin assembly factor CAF-1 has been biochemically well-characterized to deposit histones onto newly synthesized DNA. To gain insights into the in vivo functions of CAF-1 in Drosophila, we generated null mutants of the largest subunit of dCAF-1, dCAF-1-p180. We show that, unlike CAF-1 mutant yeast, dCAF-1-p180 mutant flies are hemizygous lethal. Removal of maternal dCAF-1-p180 activity by germline clones blocks oogenesis. Tissue-specific deletion of dCAF-1-p180 in the eye primordia disrupts eye development. In addition, reduction of dCAF-1-p180 activity suppresses gene silencing at heterochromatin and antagonizes Polycomb-mediated cell fate determination. Furthermore, heterozygous dCAF-1-p180 mutant flies display an increased sensitivity to gamma-irradiation and a reduced efficiency in recombinational double strand break (DSB) repair. Our experiments also show that human hCAF-1-p150 can rescue the dCAF-1-p180 mutant flies, demonstrating a functional conservation of eukaryotic CAF-1 activities in vivo. Together, our results establish that dCAF-1-p180 is an essential gene for Drosophila development and further underscore the importance of dCAF-1 in regulating gene expression and DNA repair in vivo.

CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. Publishing Authors By Initials

Y Song,F He,G Xie,X Guo,Y Xu,Y Chen,X Liang,I Stagljar,D Egli,J Ma,R Jiao,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Developmental biology

VOLUME: 311

Page Numbers: 213-22

Journal Abbreviation: Dev. Biol.

ISSN: 1095-564X

DAY: 29

MONTH: 08

YEAR: 2007

CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. Information

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LANGUAGE: eng

NlmUniqueID: 372762

CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. Information

Substance Name: Transcription Factors

Registry Number: 0

Grant and Affiliation Information for CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory.

AFFILIATION: National Laboratory of Biomacromolecules and State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, The Chinese Academy of Sciences, Datun Road 15, Chaoyang District, Beijing 100101, China.

Country: United States

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MEDLINETA: Dev Biol

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