Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression.

Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression. Research Abstract Details 

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Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression. Abstract Text:

Markus Gorfer,Sylvia Klaubauf,Dragana Bandian,Joseph Strauss,

Hygromycin B resistance was transferred to the sterile mycelia of Cadophora finlandia and Phialocephala fortinii by co-cultivation with Agrobacterium tumefaciens. Constitutively expressed green fluorescent protein (GFP) was also introduced using the same vector. Confocal laser scanning microscopy (CLSM) revealed strong fluorescence of transformants. Both traits were mitotically stable during one year of subculturing on non-selective growth medium. Southern blot analysis showed that the majority of the transformants contained single-copy integrations at random sites in the genome.

Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression. Publishing Authors By Initials

M Gorfer,S Klaubauf,D Bandian,J Strauss,

For similar genetic processes: recombination, genetic: transformation, genetic research abstracts see: genetic processes: recombination, genetic: transformation, genetic research

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Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Mycological research

VOLUME: 111

Page Numbers: 850-5

Journal Abbreviation: Mycol. Res.

ISSN: 0953-7562

DAY: 18

MONTH: 05

YEAR: 2007

Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression. Information

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LANGUAGE: eng

NlmUniqueID: 8913481

Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression. Keywords Mesh Terms:

KEYWORDS: Transformation, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression. Information

Substance Name: Hygromycin B

Registry Number: 31282-04-9

Grant and Affiliation Information for Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression.

AFFILIATION: Fungal Genomics Unit, Austrian Research Centres and BOKU Vienna, Muthgasse 18, 1190 Vienna, Austria. markus.gorfer@boku.ac.at

Country: England

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MEDLINETA: Mycol Res

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