Cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from Pseudomonas ovalis.

Cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from Pseudomonas ovalis. Research Abstract Details 

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Cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from Pseudomonas ovalis. Abstract Text:

F Yamakura,K Suzuki,

The Cd-, Cr-, and Mn-substituted enzymes of iron-superoxide dismutase from Pseudomonas ovalis were prepared from the apoenzyme by using the alkaline treatment method described before (1) with a slight modification. The Cd-substituted enzyme had 1.16 g atoms of Cd per mol of enzyme and no visible absorption. The Cr-substituted enzyme had 1.27 g atoms of Cr per mol of enzyme and had absorption maxima at 530 nm and 670 nm with a shoulder around 370 nm. The Mn-substituted enzyme had 1.27 g atoms of Mn per mol of enzyme and had absorption shoulders around 470 nm and 600 nm. The Cd-, Cr-, and Mn-substituted enzymes had no enzymatic activity. The reactivity of the four sulfhydryl groups and circular dichroism spectra of the Cd-, Cr-, and Mn-substituted enzymes were similar to those of the Fe-reconstituted enzyme. These results indicate that these metals may bind to the same site as Fe in the enzyme. The fluorescence emission intensities of the Cd-, Cr-, and Mn-substituted enzymes were 0.75, 0.46, and 0.69 times that of the apoenzyme, respectively.

Cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from Pseudomonas ovalis. Publishing Authors By Initials

F Yamakura,K Suzuki,

For similar enzymes and coenzymes: enzymes: oxidoreductases: superoxide dismutase research abstracts see: enzymes and coenzymes: enzymes: oxidoreductases: superoxide dismutase research

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Cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from Pseudomonas ovalis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 88

Page Numbers: 191-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1980

Cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from Pseudomonas ovalis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from Pseudomonas ovalis. Keywords Mesh Terms:

KEYWORDS: Superoxide Dismutase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cadmium, chromium, and manganese replacement for iron in iron-superoxide dismutase from Pseudomonas ovalis. Information

Substance Name: Superoxide Dismutase

Registry Number: EC 1.15.1.1

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Country: JAPAN

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MEDLINETA: J Biochem

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