Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine.

Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine. Research Abstract Details 

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Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine. Abstract Text:

B A Perrino,B A Martin,

It has been proposed that N-terminal myristoylation of calcineurin B is necessary for the membrane association of calcineurin. We tested the effects of Ca(2+) and myristoylation on the binding of calcineurin B alone or heterodimeric calcineurin to phosphatidylserine or phosphatidylcholine vesicles. In the presence of excess phosphatidylserine, 50-60% of total calcineurin associated with phosphatidylserine in a Ca(2+)-sensitive manner. Calcineurin did not associate with phosphatidylcholine. Calcineurin containing both the alpha and beta catalytic subunit isoforms bound to phosphatidylserine. Calmodulin interfered with the association of calcineurin with phosphatidylserine. In the presence of Ca(2+), myristoylated calcineurin B alone did not bind to phosphatidylcholine but did bind to phosphatidylserine, although to a lesser extent than the calcineurin heterodimer. Non-myristoylated calcineurin B alone, or calcineurin containing non-myristoylated calcineurin B did not associate with phosphatidylserine in the presence of Ca(2+). These results indicate: (i) Both isoforms of calcineurin bind to phosphatidylserine. (ii) A phospholipid binding site is located on the calcineurin B subunit. (iii) Calcineurin B myristoylation is required for the Ca(2+)-sensitive binding of calcineurin to phosphatidylserine vesicles in vitro.

Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine. Publishing Authors By Initials

BA Perrino,BA Martin,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biochemistry

VOLUME: 129

Page Numbers: 835-41

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 2001

Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine. Information

Substance Name: Calcineurin

Registry Number: EC 3.1.3.-

Grant and Affiliation Information for Ca(2+)- and myristoylation-dependent association of calcineurin with phosphatidylserine.

AFFILIATION: Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA. perrino@physio.unr.edu

Country: Japan

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MEDLINETA: J Biochem

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