C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation.

C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. Research Abstract Details 

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C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. Abstract Text:

N Okumura,K Takimoto,M Okada,H Nakagawa,

With purified preparations of basic fibroblast growth factor (bFGF), we studied the effect of its growth-promoting activity on C6 glioma cells. We also examined with its antibody whether the cultured glioma cells could produce it. It was shown that bFGF stimulated the DNA synthesis and proliferation of C6 glioma cells in serum-free medium, and that the activity was potentiated by heparin, the bFGF concentrations for half-maximal stimulation being 0.2 and 5 ng/ml in the presence and absence of heparin, respectively. This effect of heparin was dose-dependent and was half-maximal at 0.5 microgram/ml. Next, we raised the antiserum against bFGF and detected a single immunoreactive band from extracts of C6 glioma cells by immunoblot analysis. The immunoreactive substance was partially purified on a heparin-Sepharose column and was shown to stimulate the DNA synthesis of C6 glioma cells. On the basis of its immunoreactivity, molecular weight, affinity for heparin, and growth-promoting activity, this substance was identified as bFGF. The content of bFGF in the cells was elevated as the cell density increased, but no immunoreactivity was detected in the conditioned medium of the cells. These results suggest that C6 glioma cells produce and store bFGF which is potent in stimulating their own growth.

C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. Publishing Authors By Initials

N Okumura,K Takimoto,M Okada,H Nakagawa,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

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C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 106

Page Numbers: 904-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1989

C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. Information

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LANGUAGE: eng

NlmUniqueID: 376600

C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: metabolism

Chemical & Substance for Abstract: C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. Information

Substance Name: Heparin

Registry Number: 9005-49-6

Grant and Affiliation Information for C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation.

AFFILIATION: Institute for Protein Research, Osaka University.

Country: JAPAN

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MEDLINETA: J Biochem

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