Buffering airway acid decreases exhaled nitric oxide in asthma.

Buffering airway acid decreases exhaled nitric oxide in asthma. Research Abstract Details 

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Buffering airway acid decreases exhaled nitric oxide in asthma. Abstract Text:

Benjamin Gaston,Robin Kelly,Peter Urban,Lei Liu,Edward M Henderson,Allan Doctor,W Gerald Teague,Anne Fitzpatrick,Serpil Erzurum,John F Hunt,

BACKGROUND: The human airway is believed to be acidified in asthma. In an acidic environment nitrite is converted to nitric oxide (NO). OBJECTIVE: We hypothesized that buffering airway lining fluid acid would decrease the fraction of exhaled NO (F(ENO)). METHODS: We treated 28 adult nonsmoking subjects (9 healthy control subjects, 11 subjects with mild intermittent asthma, and 8 subjects with persistent asthma) with 3 mL of 10 mmol/L phosphate buffered saline (PBS) through a nebulizer and then serially measured F(ENO) levels. Six subjects also received PBS mouthwash alone. RESULTS: F(ENO) levels decreased after buffer inhalation. The maximal decrease occurred between 15 and 30 minutes after treatment; F(ENO) levels returned to pretreatment levels by 60 minutes. The decrease was greatest in subjects with persistent asthma (-7.1 +/- 1.0 ppb); this was more than in those with either mild asthma (-2.9 +/- 0.3 ppb) or healthy control subjects (-1.7 +/- 0.3 ppb, P < .001). Levels did not decrease in subjects who used PBS mouthwash. CONCLUSION: Neutralizing airway acid decreases F(ENO) levels. The magnitude of this change is greatest in persistent asthma. These data suggest that airway pH is a determinant of F(ENO) levels downstream from NO synthase activation. CLINICAL IMPLICATIONS: Airway biochemistry modulates F(ENO) levels. For example, nitrite is converted to NO in the airway, particularly the inflamed airway, by means of acid-based chemistry. Thus airway pH should be considered in interpreting clinical F(ENO) values. In fact, PBS challenge testing integrates airway pH and F(ENO) analysis, potentially improving the utility of F(ENO) as a noninvasive test for the type and severity of asthmatic airway inflammation.

Buffering airway acid decreases exhaled nitric oxide in asthma. Publishing Authors By Initials

B Gaston,R Kelly,P Urban,L Liu,EM Henderson,A Doctor,WG Teague,A Fitzpatrick,S Erzurum,JF Hunt,

For similar chlorides: sodium chloride research abstracts see: chlorides: sodium chloride research

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Buffering airway acid decreases exhaled nitric oxide in asthma. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of allergy and clinical immunology

VOLUME: 118

Page Numbers: 817-22

Journal Abbreviation: J. Allergy Clin. Immunol.

ISSN: 0091-6749

DAY: 1

MONTH: 09

YEAR: 2006

Buffering airway acid decreases exhaled nitric oxide in asthma. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1275002

Buffering airway acid decreases exhaled nitric oxide in asthma. Keywords Mesh Terms:

KEYWORDS: Sodium Chloride

MESH TERMS: administration & dosage

Chemical & Substance for Abstract: Buffering airway acid decreases exhaled nitric oxide in asthma. Information

Substance Name: Sodium Chloride

Registry Number: 7647-14-5

Grant and Affiliation Information for Buffering airway acid decreases exhaled nitric oxide in asthma.

AFFILIATION: Department of Pediatric Respiratory Medicine, The University of Virginia School of Medicine, Charlottesville, VA 22908, USA. bmg3g@virginia.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL 69170

ACRONYM: HL

MEDLINETA: J Allergy Clin Immunol

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