Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease.

Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease. Research Abstract Details 

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Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease. Abstract Text:

Beth A Bauer,Susan Boedges,Cristi R Cook,Elizabeth C Bryda,Craig L Franklin,

The SPRD-Pkdr1 rat model is widely used for the study of human autosomal dominant polycystic kidney disease. This rat model carries the Cy allele of the Pkdr1 gene, which results in polycystic kidney disease. Because the Cy allele is lethal in the homozygous state at weanling age, the breeding colony must be maintained in the heterozygous state. A random breeding scheme in which production of homozygous pups with enlarged kidneys indicates heterozygous breeders is commonly used. This study was performed to determine whether biochemical markers (blood urea nitrogen [BUN] or creatinine), ultrasonography, or genetic analysis could be used to select breeding animals in the SPRD-Pkdr1/Rrrc colony and thus replace the random breeding scheme with a more efficient selective breeding scheme. BUN was predictive of the Cy allele in 8- to 9-wk-old male but not female rats. Ultrasonography identified animals with polycystic kidney disease in both sexes by 9 wk of age. Microsatellite marker polymorphism analysis could not be used to determine carrier status for the Cy allele, but restriction fragment length polymorphism analysis appropriately detected the Cy allele in 100% of the animals examined. In conclusion, multiple methods can be used for detecting the Cy allele, making possible a selective breeding scheme that markedly reduces the necessary number of breeder animals and eliminates the euthanasia of offspring needed with a random test-mating scheme.

Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease. Publishing Authors By Initials

BA Bauer,S Boedges,CR Cook,EC Bryda,CL Franklin,

For similar proteins: carrier proteins: membrane transport proteins: ion channels: calcium channels: trpp cation channels research abstracts see: proteins: carrier proteins: membrane transport proteins: ion channels: calcium channels: trpp cation channels research

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Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Comparative medicine

VOLUME: 57

Page Numbers: 193-9

Journal Abbreviation: Comp. Med.

ISSN: 1532-0820

DAY: 3

MONTH: Apr

YEAR: 2007

Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease. Information

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LANGUAGE: eng

NlmUniqueID: 100900466

Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease. Keywords Mesh Terms:

KEYWORDS: TRPP Cation Channels

MESH TERMS: chemistry

Chemical & Substance for Abstract: Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease. Information

Substance Name: Creatinine

Registry Number: 60-27-5

Grant and Affiliation Information for Breeding colony refinement through phenotypic and genotypic characterization of the SPRD-Pkdr1/Rrrc rat model of polycystic kidney disease.

AFFILIATION: Research Animal Diagnostic Laboratory, University of Missouri, Columbia, MO, USA. bauerbe@missouri.edu

Country: United States

AGENCY: United States NCRR

GRANT: P40 RR16939

ACRONYM: RR

MEDLINETA: Comp Med

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