Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways.

Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways. Abstract Text:

Jia Shen,Siewting Yip,Zhixing Wang,Wei Wang,Dongming Xing,Lijun Du,

Brazilein (6a,7-dihydro-3,6a,10-trihydroxy-benz[b]indeno[1,2-d]pyran-9(6H)-one) is a compound isolated from Caesalpinia sappan. The vasoactivities of brazilein were evaluated in isolated rat thoracic aorta. The results showed that brazilein can dose-dependently induce contraction of rat thoracic aorta in the resting and phenylephrine pre-evoked state. The average response to 100 muM of brazilein was 30% of the 50 mM KCl contraction, 26% of the 10 muM phenylephrine and 116% of the 20 mM caffeine contraction in comparison. The effects of vasocontraction were proved not to be endothelial dependent and could not be inhibited by alpha-adrenergic receptor blocker phentolamine, beta-adrenergic receptor blocker propranolol, M-adrenaline receptor blocker atropine, angiotensin II receptor blocker losartan or the non-selective nitric oxide synthase (NOS) inhibitor NG-Nitro-l-Arginine Methyl Ester (l-NAME). However the influx of extracellular calcium seemed to be required for this action, because depletion of extracellular calcium and the addition of L-type calcium ion channel antagonist (nimodipine and diltiazem), calcium ion channel activator (BAY-K8644) and potassium ion channel opener (pinacidil) could significantly affect the contraction induced by brazilein. We also investigated the possible signal mechanisms underlying brazilein-induced contraction using selective inhibitors. The inhibitors of myosin light chain kinase (MLCK), Rho-kinase (ROK) and extracellular signal regulated kinase (ERK) can suppress the effect of brazilein respectively, whereas inhibitors of other signaling or receptor molecules such as protein kinase C (PKC) and inositol 1,4,5-triphosphate (IP(3)) receptor had no effect. All these results demonstrated that brazilein can induce contraction of rat aorta, that the Ca(2+) influx, ROK and ERK signal pathways and MLCK activation must be involved in the contractile processes.

Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways. Publishing Authors By Initials

J Shen,S Yip,Z Wang,W Wang,D Xing,L Du,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: European journal of pharmacology

VOLUME: 580

Page Numbers: 366-71

Journal Abbreviation: Eur. J. Pharmacol.

ISSN: 0014-2999

DAY: 17

MONTH: 11

YEAR: 2007

Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1254354

Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca(2+) entry and ROK, ERK pathways.

AFFILIATION: Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Eur J Pharmacol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Brazilein-induced contraction of rat arterial smooth muscle involves activation of Ca2+ entry and ROK, ERK pathways Related Publications

• Inhibitory effects of blocking voltage-dependent potassium channel 1.3 on human monocyte-derived macrophage differentiation into foam cells.
• Measurement of retinal thickness in normal subjects with retinal thickness analyzer.
• Oxidation of plasma proteins is not increased after supplementation with eicosapentaenoic and docosahexaenoic acids.
• [A research of endothelial cell-targeted therapy for cure of hypertrophic scar]
• [Choice of liquid resuscitation in critical patients]
• [Current state of diagnosis and treatment of incomplete Kawasaki disease]
• [Detection of a new mutation (1343-TT) in the iduronate-2-sulfatase gene from a Chinese patient with mucopolysaccharidosis type II]
• [Detection of a new mutation (T1140C)in a Chinese Guangdong patient with hunter syndrome]
• [Dynamic change of metabolism related protein in liver tissue of rats' model of hepatic fibrosis and regulatory effect of fuzheng huayu decoction on it]
• [Effect of adrenomedullin1-50 on chronic hypoxic pulmonary hypertension in rats]
• [Effect of alginate purity on microencapsulated hepatocyte overgrowth in peritoneal transplantation]
• [Effect of coronal preflaring on the instrumentation of curved premolar canals.]
• [Effect of gamma-aminobutyric acid B receptor on nitric oxide/nitric oxide synthase system during recurrent febrile seizures]
• [Effect of the width of excisional mucosa stoma ring on postoperative results of procedure for prolapse and hemorrhoids method]
• [Effects of urapidil and cimetidine on wedged hepatic venous pressure and systemic hemodynamics in cirrhotic patients with portal hypertension]
• [Experimental study on early escharectomy after major burns in minipigs]
• [Factors influencing isolation and cloning of chicken primordial germ cells]
• [Further improve the diagnostic and therapeutic level for Kawasaki disease]
• [Inhibitory effect of blocking MaxiK channel on human monocyte-derived macrophages differentiation into foam cells]
• [Measure for human motion parameters based on model]
• [Modified Mitchell's technique for repair epispadias with a report of 3 cases]
• [Present status and prospects of development of neonatology in China]
• [Role of gasotransmitters in the pathogenesis of pulmonary hypertension]
• [Sequence analysis of 5' non-coding region of hepatitis G viruses and preliminary classification of genotypes]
• [Survey of plasma lipid value in children in Beijing area and the changes of gaseous molecule-hydrogen sulfide in the ones with dyslipidemia]
• [Survey research of serum anti-influenza virus antibody levels in Chinese young people during 1997-2000]
• [The exposure level of microwave radiation for the workers enclosed in metal separation rooms in the workplace]
• [The proapoptotic effect of the homogenate of the tissue of different parts of pig's full thickness dermal wounds on cultured fibroblasts]
• [UDP-glucuronosyltransferase 1A1 gene and neonatal jaundice]
• [miRNA: a new regulator of gene expression]