Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast.

Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast. Research Abstract Details 

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Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast. Abstract Text:

Youn Uck Kim,Hisashi Ashida,Kenichiro Mori,Yusuke Maeda,Yeongjin Hong,Taroh Kinoshita,Youn Uck Kim,Hisashi Ashida,Kenichiro Mori,Yusuke Maeda,Yeongjin Hong,Taroh Kinoshita,

GPI mannosyltransferase I (GPI-MT-I) transfers the first mannose to a GPI-anchor precursor, glucosamine-(acyl)phosphatidylinositol [GlcN-(acyl)PI]. Mammalian GPI-MT-I consists of two components, PIG-M and PIG-X, which are homologous to Gpi14p and Pbn1p in Saccharomyces cerevisiae, respectively. In the present study, we disrupted yeast GPI14 and analysed the phenotype of gpi14 yeast. The gpi14 haploid cells were inviable and accumulated GlcN-(acyl)PI. We cloned PIG-M homologues from human, Plasmodium falciparum (PfPIG-M) and Trypanosoma brucei (TbGPI14), and tested whether they could complement gpi14-disrupted yeast. None of them restored GPI-MT-I activity and cell growth in gpi14-disrupted yeast. However, gpi14-disrupted yeast cells with human PIG-M, but not with PfPIG-M or TbGPI14, grew slowly but significantly when they were supplemented with rat PIG-X. This suggests that the association of PIG-X and PIG-M for GPI-MT-I activity is not interchangeable between mammals and the other lower eukaryotes.

Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast. Publishing Authors By Initials

YU Kim,H Ashida,K Mori,Y Maeda,Y Hong,T Kinoshita,YU Kim,H Ashida,K Mori,Y Maeda,Y Hong,T Kinoshita,

For similar animals: invertebrates: protozoa: sarcomastigophora: mastigophora: zoomastigophora: kinetoplastida: trypanosomatina: trypanosoma: trypanosoma brucei brucei research abstracts see: animals: invertebrates: protozoa: sarcomastigophora: mastigophora: zoomastigophora: kinetoplastida: trypanosomatina: trypanosoma: trypanosoma brucei brucei research

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Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 142

Page Numbers: 123-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 22

MONTH: 05

YEAR: 2007

Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast. Keywords Mesh Terms:

KEYWORDS: Trypanosoma brucei brucei

MESH TERMS: enzymology

Chemical & Substance for Abstract: Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast. Information

Substance Name: glycosylphosphatidylinositol mannosyltra

Registry Number: EC 2.4.1.-

Grant and Affiliation Information for Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast.

AFFILIATION: Department of Biological Sciences, Sun Moon University, A-San City, Korea. kimyu@sunmoon.ac.kr

Country: Japan

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MEDLINETA: J Biochem (Tokyo)

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