Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold.

Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. Research Abstract Details 

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Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. Abstract Text:

Natsuko Kakudo,Ayuko Shimotsuma,Shogo Miyake,Satoshi Kushida,Kenji Kusumoto,Natsuko Kakudo,Ayuko Shimotsuma,Shogo Miyake,Satoshi Kushida,Kenji Kusumoto,Natsuko Kakudo,Ayuko Shimotsuma,Shogo Miyake,Satoshi Kushida,Kenji Kusumoto,

Human adipose-derived stem cells (ASCs) have the capacity to regenerate and the potential to differentiate into multiple lineages of mesenchymal cells. The aim of this study was to investigate the possibility of using honeycomb collagen scaffold to culture ASCs in bone tissue engineering. The osteogenic capacity of ASCs in vitro, was confirmed by histology and measuring the expression of cbfa-1. After that, ASCs were cultured for up to 14 days in the honeycomb scaffold to allow a high density, three-dimensional culture. Scanning electron microscopy data showed that the scaffold was filled with the grown ASCs, and calcification, stained black with von Kossa, was confirmed. Furthermore, The ASC-loaded honeycomb collagen scaffolds cultured for 14 days were subcutaneously transplanted into nude mice, and excised after 8 weeks. Bone formation in vivo was examined using HE stain, von Kossa stain, and osteocalcin immunostain. Those histological views showed significant positive stains in the samples of osteogenic medium in the three types of stain. These results suggest that this carrier is a suitable scaffold for ASCs and will be useful as a three-dimensional bone tissue engineering scaffold in vitro and in vivo. (c) 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008.

Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. Publishing Authors By Initials

N Kakudo,A Shimotsuma,S Miyake,S Kushida,K Kusumoto,N Kakudo,A Shimotsuma,S Miyake,S Kushida,K Kusumoto,N Kakudo,A Shimotsuma,S Miyake,S Kushida,K Kusumoto,

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Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical materials research. Part A

VOLUME: 84

Page Numbers: 191-7

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ISSN: 1549-3296

DAY: 26

MONTH: Jan

YEAR: 2008

Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. Information

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LANGUAGE: eng

NlmUniqueID: 101234237

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Grant and Affiliation Information for Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold.

AFFILIATION: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Osaka, Japan.

Country: United States

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MEDLINETA: J Biomed Mater Res A

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