Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea.

Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea. Research Abstract Details 

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Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea. Abstract Text:

R R Mohan,W J Kim,R R Mohan,L Chen,S E Wilson,

PURPOSE: To examine the expression of transforming growth factor family members bone morphogenic proteins 2 and 4 (BMP2, BMP4), their receptor mRNAs, and BMP2 and BMP4 proteins in the cells of the human cornea. The effects of BMP2 and BMP4 on corneal fibroblast proliferation and apoptosis were also examined. METHODS: Reverse transcription-polymerase chain reaction, immunoprecipitation, and western blot analysis were used to examine mRNA and protein expression in cultured human corneal cells. Immunocytochemistry was applied to examine protein localization in fresh frozen human cornea cells. Stimulation and inhibition of nuclear factor-kappaB (NF-kappaB) activation was evaluated by gel shift assay. Apoptosis was examined using trypan blue exclusion, laddering of DNA, CPP32 assay, and transmission electron microscopy. Proliferation was monitored by counting cells. RESULTS: BMP2 and BMP4 mRNAs and proteins were expressed in cultured human corneal epithelial cells, keratocytes, and corneal endothelial cells. BMP2 and BMP4 were detected in each major corneal cell type in fresh frozen human cornea. BMP receptor IA, IB, and II mRNAs were also detected in cultured human corneal epithelial cells, keratocytes, and endothelial cells. BMP2 and BMP4 stimulated activation of NF-kappaB. Actinomycin D and SN50 peptide, but not SN50M control peptide, inhibited NF-kappaB activation in response to BMP2 or BMP4. BMP2 and BMP4 stimulated apoptosis of corneal fibroblast cells when NF-kappaB activation was inhibited with the nonselective inhibitor actinomycin D or selective inhibitor SN50. The nonsteroidal anti-inflammatory agents ketorolac tromethamine and diclofenac sodium augmented the effect of BMP2 on corneal fibroblast apoptosis. BMP2 and BMP4 both stimulated proliferation of corneal fibroblast cells in the absence of inhibitors of NF-kappaB activation. CONCLUSIONS: BMP2, BMP4, and their receptors are expressed in the cells of the adult human cornea. The functions regulated by these cytokines may include keratocyte proliferation and apoptosis.

Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea. Publishing Authors By Initials

RR Mohan,WJ Kim,RR Mohan,L Chen,SE Wilson,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Investigative ophthalmology & visual science

VOLUME: 39

Page Numbers: 2626-36

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 19

MONTH: Dec

YEAR: 1998

Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7703701

Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea. Information

Substance Name: Bone Morphogenetic Protein Receptors

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Bone morphogenic proteins 2 and 4 and their receptors in the adult human cornea.

AFFILIATION: Eye Institute and the Department of Cell Biology, The Cleveland Clinic Foundation, Ohio, USA.

Country: UNITED STATES

AGENCY: United States NEI

GRANT: EY10056

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

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