Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts.

Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts. Research Abstract Details 

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Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts. Abstract Text:

R C Pereira,A N Economides,E Canalis,

Bone morphogenetic proteins (BMP) induce the differentiation of cells of the osteoblastic lineage and enhance the function of the osteoblast. Growth factor activity is regulated by binding proteins, and we previously showed that BMPs induce noggin, a glycoprotein that binds and blocks BMP action. Recently, additional BMP antagonists, such as gremlin, have been described, but there is no information about their expression or function in osteoblasts. We tested for the expression of gremlin and studied its induction by BMPs in cultures of osteoblast-enriched cells from 22-day-old fetal rat calvariae (Ob cells). BMP-2 caused a time- and dose-dependent increase in gremlin messenger RNA and polypeptide levels, as determined by Northern and Western blot analyses. The effects of BMP-2 on gremlin transcripts were independent of new protein synthesis. BMP-2 increased the rate of gremlin transcription as determined by nuclear run-on assays. Fibroblast growth factor-2 and platelet-derived growth factor BB also induced gremlin, but other hormones and growth factors had no effect. Gremlin prevented the stimulatory effects of BMP-2 on DNA, collagen, noncollagen protein synthesis, and alkaline phosphatase activity in Ob cells. In conclusion, BMPs induce gremlin transcription in Ob cells, a mechanism that probably limits BMP action in osteoblasts.

Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts. Publishing Authors By Initials

RC Pereira,AN Economides,E Canalis,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Endocrinology

VOLUME: 141

Page Numbers: 4558-63

Journal Abbreviation: Endocrinology

ISSN: 0013-7227

DAY: 19

MONTH: Dec

YEAR: 2000

Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts. Information

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LANGUAGE: eng

NlmUniqueID: 375040

Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: analysis

Chemical & Substance for Abstract: Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Bone morphogenetic proteins induce gremlin, a protein that limits their activity in osteoblasts.

AFFILIATION: Department of Research, Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105, USA.

Country: UNITED STATES

AGENCY: United States NIAMS

GRANT: AR-21707

ACRONYM: AR

MEDLINETA: Endocrinology

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