Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells.

Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. Abstract Text:

Guozhi Xiao,Rajaram Gopalakrishnan,Di Jiang,Elizabeth Reith,M Douglas Benson,Renny T Franceschi,

Osteoblasts secrete a complex extracellular matrix (ECM) containing collagenous and noncollagenous proteins, bone morphogenetic proteins (BMPs), and growth factors. Osteoblast-specific gene expression requires ascorbic acid (AA)-dependent assembly of a collagenous ECM. Matrix responsiveness requires an alpha2beta1 integrin-collagen interaction and mitogen-activated protein kinase (MAPK) activity, which phosphorylates and activates the osteoblast-specific transcription factor Cbfa1. This study examines interactions between this integrin/MAPK-mediated pathway and signals initiated by BMPs contained in the osteoblast matrix. MC3T3-E1 cells were shown to constitutively express BMP-2, BMP-4, and BMP-7. Noggin, a specific BMP inhibitor, reversibly blocked AA-induced gene expression, indicating that BMP production by MC3T3-E1 cells was necessary for differentiation. The ability of exogenously added BMP-2, BMP-4, or BMP-7 to stimulate osteocalcin (OCN) and bone sialoprotein (BSP) mRNAs or OCN promoter activity was synergistically increased in cells that were actively synthesizing an ECM (i.e., were grown in the presence of AA). A minimum of 4 days of ECM accumulation was required for this synergistic response to be observed. Neither BMP-7, AA, nor a combination of these two treatments had major effects on Cbfa1 messenger RNA (mRNA) or protein levels, as would be expected if regulation was mainly at the posttranscriptional level. U0126, a specific inhibitor of MAPK/extracellular signal-regulated kinase (MEK), blocked AA- or BMP-7/AA-dependent gene expression in a time- and dose-dependent manner that was closely correlated with inhibition of extracellular signal-regulated kinase (ERK) phosphorylation. This work establishes that autocrine BMP production as well as integrin-mediated cell-collagen interactions are both required for osteoblast differentiation, and both these pathways require MAP kinase activity.

Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. Publishing Authors By Initials

G Xiao,R Gopalakrishnan,D Jiang,E Reith,MD Benson,RT Franceschi,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bone and mineral research : the officia

VOLUME: 17

Page Numbers: 101-10

Journal Abbreviation: J. Bone Miner. Res.

ISSN: 0884-0431

DAY: 19

MONTH: Jan

YEAR: 2002

Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8610640

Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: genetics

Chemical & Substance for Abstract: Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. Information

Substance Name: Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells.

AFFILIATION: Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor 48109-1078, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: DE 11723

ACRONYM: DE

MEDLINETA: J Bone Miner Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells Related Publications

• A genetic-demographic approach reveals male-specific association between survival and tumor necrosis factor (a/g)-308 polymorphism.
• A novel mitochondrial DNA-like sequence insertion polymorphism in Intron I of the FOXO1A gene.
• Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation.
• Back to the future: the ribosome as an antibiotic target.
• Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells.
• Chapter 27: Research needs following initial licensure of virus-like particle HPV vaccines.
• Common evolutionary origin of the immune and neuroendocrine systems: from morphological and functional evidence to in silico approaches.
• Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development.
• Daunorubicin and daunorubicinol tissue concentrations in gastric cancer patients after local administration of a liposomal preparation.
• Different types of cell death in organismal aging and longevity: state of the art and possible systems biology approach.
• Erythrocyte plasma membranes obtained from centenarians show different functional properties.
• Healing Cranial Defects with AdRunx2-transduced Marrow Stromal Cells.
• Human longevity within an evolutionary perspective: The peculiar paradigm of a post-reproductive genetics.
• Human papillomavirus infection in women with and without cervical cancer in Warsaw, Poland.
• Human papillomavirus types among women infected with HIV: a meta-analysis.
• Immunoproteasome in Macaca fascicularis: No Age-Dependent Modification of Abundance and Activity in the Brain and Insight into an in silico Structural Model.
• In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation.
• Incidence of primary liver cancer in Italy between 1988 and 2002: An age-period-cohort analysis.
• Inflammaging as a major characteristic of old people: can it be prevented or cured?
• Long-term immune-endocrine effects of bereavement: relationships with anxiety levels and mood.
• Members of the human papillomavirus type 18 family (alpha-7 species) share a common association with adenocarcinoma of the cervix.
• New molecular targets and novel anticancer treatments: emerging trends in neuro-oncology.
• Paraoxonase 1: genetics and activities during aging.
• Plasma concentrations of interleukin-1-beta, interleukin-6 and tumor necrosis factor-alpha, and of their soluble receptors and receptor antagonist in anorexia nervosa.
• Prevalence of hepatitis B and C serological markers among first-time blood donors in Brazil: a multi-center serosurvey.
• Risk of cancer following immunosuppression in organ transplant recipients and in HIV-positive individuals in southern Europe.
• Serologic response to oncogenic human papillomavirus types in male and female university students in Busan, South Korea.
• The IARC commitment to cancer prevention: the example of papillomavirus and cervical cancer.
• Use of a stringent dimerizer-regulated gene expression system for controlled BMP2 delivery.
• Usefulness of the comprehensive geriatric assessment in older patients with upper gastrointestinal bleeding: a two-year follow-up study.