Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells.

Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells. Research Abstract Details 

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Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells. Abstract Text:

S Marrony,F Bassilana,K Seuwen,H Keller,

Bone-forming osteoblasts differentiate from pluripotent mesenchymal stem cells (MSCs) in a multistage process that can be modeled in vitro using MSCs isolated from adult human trabecular bone or bone marrow. To identify new genes involved in osteoblast differentiation, we have performed large-scale gene expression profiling using high-density cDNA microarrays in primary human MSCs treated with the known osteogenic agent bone morphogenetic protein 2 (BMP-2). The vascular endothelial growth factor (VEGF) family member placental growth factor (PlGF) was found as an early regulated gene whose induction was already detected after 2 h treatment with BMP-2. Tissue distribution analysis of PlGF mRNA expression using microarrays revealed a very restricted expression of PlGF only in BMP-2-treated MSCs and in placenta as expected. Ribonuclease protection assay (RPA) confirmed the induction of PlGF and showed preferential expression of the PlGF-1 isoform over PLGF-2 in MSCs and MG63 cells. BMP-2 stimulated PlGF expression in MG63 cells with an EC50 of about 50 ng/ml and mRNA levels peaked between 24 and 32 h after stimulation. Furthermore, induction of PlGF by BMP-2 appeared specific, as other osteogenic agents including vitamin D3, transforming growth factor beta, and basic fibroblast growth factor were inactive. BMP-2 stimulated PlGF secretion from MG63 and MSC cells, but PlGF had no effect on MSC proliferation and osteoblastic differentiation. Based on the known function of PlGF in the recruitment of endothelial and hematopoietic stem cells, these results suggest a paracrine role for MSC-derived PlGF in the angiogenesis and hematopoiesis that accompany BMP-2-induced bone formation.

Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells. Publishing Authors By Initials

S Marrony,F Bassilana,K Seuwen,H Keller,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Bone

VOLUME: 33

Page Numbers: 426-33

Journal Abbreviation: Bone

ISSN: 8756-3282

DAY: 19

MONTH: Sep

YEAR: 2003

Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 8504048

Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: physiology

Chemical & Substance for Abstract: Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells. Information

Substance Name: product placenta growth factor

Registry Number: 144589-93-5

Grant and Affiliation Information for Bone morphogenetic protein 2 induces placental growth factor in mesenchymal stem cells.

AFFILIATION: Bone Metabolism Unit, Novartis Pharma AG, CH-4002 Basel, Switzerland.

Country: United States

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MEDLINETA: Bone

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