Bone marrow fails to differentiate into liver epithelium during murine development and regeneration.

Bone marrow fails to differentiate into liver epithelium during murine development and regeneration. Research Abstract Details 

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Bone marrow fails to differentiate into liver epithelium during murine development and regeneration. Abstract Text:

C Bart Rountree,Xuli Wang,Shundi Ge,Lora Barsky,Judy Zhu,Ignacio Gonzales,Gay M Crooks,

Recent reports have provided conflicting conclusions regarding the role for bone marrow (BM)-derived cells in the regeneration of liver. Our aim was to investigate the potential of BM to contribute to liver epithelium using different BM transplant models designed to explore differentiation during normal liver development and regeneration after toxic injury. BM cells from transgenic green fluorescent protein (GFP) mice were injected into neonatal and adult immunodeficient and neonatal immune-competent mice. Three distinct models of liver injury were employed to test the contribution of marrow to the regeneration of hepatocytes, cholangiocytes, and oval cells in immune-deficient adult animals after neonatal transplant. Immunohistochemistry was combined with flow cytometry (FACS) and reverse transcription (RT)-PCR to increase the sensitivity and specificity of the analyses. Although GFP+ marrow-derived cells were observed in the livers of all transplanted animals, immunohistochemistry failed to demonstrate any marrow derived hepatocytes or cholangiocytes. FACS confirmed that GFP+ marrow-derived cells in the liver maintained expression of CD45, a leukocyte marker. Gene expression studies of GFP+ cells isolated by FACS failed to demonstrate expression of liver specific genes in these marrow-derived cells. CONCLUSION: Through highly sensitive and specific analyses, we were unable to demonstrate any evidence of transdifferentiation of BM-derived cells into epithelial hepatic tissue during the period of rapid growth in the neonatal period. Furthermore, although increased migration of hematopoietic cells to the liver occurred after toxic injury, these cells did not contribute directly to the replacement of hepatocytes, cholangiocytes, or oval cells.

Bone marrow fails to differentiate into liver epithelium during murine development and regeneration. Publishing Authors By Initials

CB Rountree,X Wang,S Ge,L Barsky,J Zhu,I Gonzales,GM Crooks,

For similar animals: animal population groups: animals, inbred strains: mice, inbred strains: mice, inbred c57bl research abstracts see: animals: animal population groups: animals, inbred strains: mice, inbred strains: mice, inbred c57bl research

PUBMED ID PMID:

MEDLINE DATE:

Bone marrow fails to differentiate into liver epithelium during murine development and regeneration. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Hepatology (Baltimore, Md.)

VOLUME: 45

Page Numbers: 1250-60

Journal Abbreviation:

ISSN: 0270-9139

DAY: 3

MONTH: May

YEAR: 2007

Bone marrow fails to differentiate into liver epithelium during murine development and regeneration. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8302946

Bone marrow fails to differentiate into liver epithelium during murine development and regeneration. Keywords Mesh Terms:

KEYWORDS: Mice, Inbred C57BL

MESH TERMS: physiology

Chemical & Substance for Abstract: Bone marrow fails to differentiate into liver epithelium during murine development and regeneration. Information

Substance Name: 1-Naphthylisothiocyanate

Registry Number: 551-06-4

Grant and Affiliation Information for Bone marrow fails to differentiate into liver epithelium during murine development and regeneration.

AFFILIATION: Division of Gastroenterology, Hepatology, and Nutrition, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01-DK68719

ACRONYM: DK

MEDLINETA: Hepatology

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