Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1.

Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1. Research Abstract Details 

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Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1. Abstract Text:

Y Tabata,L Hong,S Miyamoto,M Miyao,N Hashimoto,Y Ikada,

The objective of the present study is to investigate the addition effect of transforming growth factor (TGF)-beta1 on the bone formation at a rabbit skull defect induced by autologous bone marrow (BM). Following application of gelatin microspheres containing TGF-beta1, with or without BM cells to skull bone defects, bone formation at the defect was assessed by soft X-ray, dual energy X-ray absorptometry (DEXA), and histological examinations. After implantation for 6 weeks, gelatin microspheres containing 0.05 microg of TGF-beta1 plus 10(6) of BM cells induced bone formation at the 6 mm diameter bone defect. The defect was histologically closed by newly formed bone tissue, whilst both gelatin microspheres containing 0.05 microg of TGF-beta1, and 10(6) and 10(7) of BM cells were ineffective. A DEXA experiment revealed that combination of gelatin microspheres containing TGF-beta1 with BM cells enhanced the bone mineral density at the skull defect to a significantly greater extent than other agents. These findings indicate that a combination of gelatin microspheres containing TGF-beta1 enabled BM cells to enhance the osteoinductive ability, resulting in bone formation even at the cell number at which BM cells alone were ineffective.

Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1. Publishing Authors By Initials

Y Tabata,L Hong,S Miyamoto,M Miyao,N Hashimoto,Y Ikada,

For similar natural sciences: physics: radiation: radiation, ionizing: x-rays research abstracts see: natural sciences: physics: radiation: radiation, ionizing: x-rays research

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Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 11

Page Numbers: 891-901

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 2000

Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1. Information

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LANGUAGE: eng

NlmUniqueID: 9007393

Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1. Keywords Mesh Terms:

KEYWORDS: X-Rays

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1. Information

Substance Name: Gelatin

Registry Number: 9000-70-8

Grant and Affiliation Information for Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1.

AFFILIATION: Institute for Frontier Medical Sciences, Kyoto University, Japan. yasuhiko@frontier.kyoto-u.ac.jp

Country: Netherlands

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MEDLINETA: J Biomater Sci Polym Ed

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