BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm.

BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm. Research Abstract Details 

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BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm. Abstract Text:

Valerie Gouon-Evans,Lise Boussemart,Paul Gadue,Dirk Nierhoff,Christoph I Koehler,Atsushi Kubo,David A Shafritz,Gordon Keller,

When differentiated in the presence of activin A in serum-free conditions, mouse embryonic stem cells efficiently generate an endoderm progenitor population defined by the coexpression of either Brachyury, Foxa2 and c-Kit, or c-Kit and Cxcr4. Specification of these progenitors with bone morphogenetic protein-4 in combination with basic fibroblast growth factor and activin A results in the development of hepatic populations highly enriched (45-70%) for cells that express the alpha-fetoprotein and albumin proteins. These cells also express transcripts of Afp, Alb1, Tat, Cps1, Cyp7a1 and Cyp3a11; they secrete albumin, store glycogen, show ultrastructural characteristics of mature hepatocytes, and are able to integrate into and proliferate in injured livers in vivo and mature into hepatocytes expressing dipeptidyl peptidase IV or fumarylacetoacetate hydrolase. Together, these findings establish a developmental pathway in embryonic stem cell differentiation cultures that leads to efficient generation of cells with an immature hepatocytic phenotype.

BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm. Publishing Authors By Initials

V Gouon-Evans,L Boussemart,P Gadue,D Nierhoff,CI Koehler,A Kubo,DA Shafritz,G Keller,

For similar biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research abstracts see: biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research

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BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature biotechnology

VOLUME: 24

Page Numbers: 1402-11

Journal Abbreviation: Nat. Biotechnol.

ISSN: 1087-0156

DAY: 5

MONTH: 11

YEAR: 2006

BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9604648

BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm. Keywords Mesh Terms:

KEYWORDS: Signal Transduction

MESH TERMS: metabolism

Chemical & Substance for Abstract: BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm. Information

Substance Name: fumarylacetoacetase

Registry Number: EC 3.7.1.2

Grant and Affiliation Information for BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm.

AFFILIATION: Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, New York 10029, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: U01 DK072513

ACRONYM: DK

MEDLINETA: Nat Biotechnol

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