BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.

BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines. Research Abstract Details 

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BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines. Abstract Text:

Tingjiao Liu,Yuhao Gao,Kei Sakamoto,Tokutaro Minamizato,Keizo Furukawa,Tomoo Tsukazaki,Yasuaki Shibata,Kazuhisa Bessho,Toshihisa Komori,Akira Yamaguchi,

To investigate the molecular mechanism underlying the differentiation of osteoblasts and chondroblasts, we established a clonal cell lines, RD-C6, from Runx2-deficient mouse embryos. RD-C6 cells expressed almost undetectable levels of phenotypes related to osteoblast and chondroblast differentiation at basal culture condition, whereas treatment with recombinant human bone morphogenetic protein-2 (rhBMP-2) or transduction of BMP-2 by adenovirus effectively induced this cell line to express mRNA related to the differentiation of osteoblasts and chondroblasts including alkaline phosphatase, osteocalcin, and osterix. Transduction of Runx2 also induced the expression of these mRNA in RD-C6 cells. BMP-2 transduction increased expression levels of mRNA for Msx2 and Dlx5, but Runx2 transduction induced no significant increases in expression levels of these mRNA. Microarray analysis using RD-C6 cells with or without rhBMP-2 treatment demonstrated that BMP-2 upregulated 66 genes including 13 transcription-related molecules such as Id1, Id2, Id4, Hey1, Smad6, Smad7, and Msx2. To confirm bone and cartilage formation ability of RD-C6 cells, we transplanted RD-C6 cells into the peritoneal cavity of athymic mice using diffusion chambers with rhBMP-2. RD-C6 cells generated unmineralized cartilage but not bone. These results indicate that BMP-2 induces Runx2-deficient cells to express markers related to osteoblast and chondroblast differentiation using a Runx2-independent pathway, but it failed to induce these cells to differentiate into bone-forming osteoblasts and mature chondrocytes.

BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines. Publishing Authors By Initials

T Liu,Y Gao,K Sakamoto,T Minamizato,K Furukawa,T Tsukazaki,Y Shibata,K Bessho,T Komori,A Yamaguchi,

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BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of cellular physiology

VOLUME: 211

Page Numbers: 728-35

Journal Abbreviation: J. Cell. Physiol.

ISSN: 0021-9541

DAY: 19

MONTH: Jun

YEAR: 2007

BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines. Information

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LANGUAGE: eng

NlmUniqueID: 50222

BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines. Keywords Mesh Terms:

KEYWORDS: Transplants

MESH TERMS: genetics

Chemical & Substance for Abstract: BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines. Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.

AFFILIATION: Section of Oral Pathology, Department of Oral Restitution, Graduate School of Tokyo Medical and Dental University, Tokyo, Japan.

Country: United States

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MEDLINETA: J Cell Physiol

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