BMP-2 induces cell migration and periostin expression during atrioventricular valvulogenesis.

BMP-2 induces cell migration and periostin expression during atrioventricular valvulogenesis. Research Abstract Details 

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BMP-2 induces cell migration and periostin expression during atrioventricular valvulogenesis. Abstract Text:

Kei Inai,Russell A Norris,Stanley Hoffman,Roger R Markwald,Yukiko Sugi,

Atrioventricular (AV) endocardium transforms into the cushion mesenchyme, the primordia of the valves and membranous septa, through epithelial-mesenchymal transformation (EMT). While bone morphogenetic protein (BMP)-2 is known to be critical for AV EMT, the role of BMP-2 in post-EMT AV valvulogenesis remains to be elucidated. To find BMP signaling loops, we first localized Type I BMP receptors (BMPRs), BMPR-1A (ALK3), -1B (ALK6) and ALK2 in AV cushion mesenchyme in stage-24 chick embryos. Based on the BMP receptor expression pattern, we examined the functional roles of BMP-2 and BMP signaling in post-EMT valvulogenesis by using stage-24 AV cushion mesenchymal cell aggregates cultured on 3D-collagen gels. Exogenous BMP-2 or constitutively active (ca) BMPR-1B (ALK6)-virus treatments induced migration of the mesenchymal cells into the collagen gels, whereas noggin, an antagonist of BMPs, or dominant-negative (dn) BMPR-1 B (ALK6)-virus treatments reduced cell migration from the mesenchymal cell aggregates. Exogenous BMP-2 or caBMPR-1B (ALK6) treatments significantly promoted expression of an extracellular matrix (ECM) protein, periostin, a known valvulogenic matrix maturation mediator, at both mRNA and protein levels, whereas periostin expression was repressed by adding noggin or dnBMPR-1B (ALK6)-virus to the culture. Moreover, transcripts of Twist and Id1, which have been implicated in cell migration in embryogenesis and activation of the periostin promoter, were induced by BMP-2 but repressed by noggin in cushion mesenchymal cell cultures. These data provide evidence that BMP-2 and BMP signaling induce biological processes involved in early AV valvulogenesis, i.e. mesenchymal cell migration and expression of periostin, indicating critical roles for BMP signaling in post-EMT AV cushion tissue maturation and differentiation.

BMP-2 induces cell migration and periostin expression during atrioventricular valvulogenesis. Publishing Authors By Initials

K Inai,RA Norris,S Hoffman,RR Markwald,Y Sugi,

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BMP-2 induces cell migration and periostin expression during atrioventricular valvulogenesis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Developmental biology

VOLUME: 315

Page Numbers: 383-96

Journal Abbreviation: Dev. Biol.

ISSN: 1095-564X

DAY: 31

MONTH: 12

YEAR: 2007

BMP-2 induces cell migration and periostin expression during atrioventricular valvulogenesis. Information

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LANGUAGE: eng

NlmUniqueID: 372762

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Grant and Affiliation Information for BMP-2 induces cell migration and periostin expression during atrioventricular valvulogenesis.

AFFILIATION: Department of Cell Biology and Anatomy and Cardiovascular Developmental Biology Center, Medical University of South Carolina, 171 Ashley Ave., Charleston, SC 29425, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL33756

ACRONYM: HL

MEDLINETA: Dev Biol

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