BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1.

BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. Abstract Text:

M K Majumdar,E Wang,E A Morris,M K Majumdar,E Wang,E A Morris,

Bone morphogenetic proteins play important roles in connective tissue morphogenesis. In this study, we used human multipotential mesenchymal cells as a target to analyze the effect of bone morphogenetic proteins on chondrogenesis. We also analyzed the effect of proinflammatory cytokine interleukin-1 on chondrogenic-differentiated cells and the interaction of IL-1beta with bone morphogenetic proteins. Cells placed in a 3-dimensional matrix of alginate beads and cultured in a serum-free media with bone morphogenetic protein-2 and -9 induced expression of type II collagen (Col2A1) mRNA and increased expression of aggrecan and cartilage oligomeric matrix protein suggesting chondrogenic differentiation of the cells. The transcription factor Sox-9 that regulates both Col2A1 and aggrecan gene expression showed increased expression with BMP treatment. Chondrogenic differentiated cells treated with interleukin-1 decreased Sox-9, Col2A1 and aggrecan gene expression. Removal of interleukin-1 and further addition of bone morphogenetic proteins resulted in returned expression of chondrogenic markers. Chondrogenic differentiated cells cultured in the presence of different concentrations of bone morphogenetic proteins and interleukin-1 showed that bone morphogenetic proteins were able to partially block the suppressive effect of interleukin-1. This study shows that bone morphogenetic proteins play an important role in chondrogenesis and may prove to be potential therapeutics in cartilage repair.

BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. Publishing Authors By Initials

MK Majumdar,E Wang,EA Morris,MK Majumdar,E Wang,EA Morris,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of cellular physiology

VOLUME: 189

Page Numbers: 275-84

Journal Abbreviation: J. Cell. Physiol.

ISSN: 0021-9541

DAY: 19

MONTH: Dec

YEAR: 2001

BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 50222

BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: genetics

Chemical & Substance for Abstract: BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. Information

Substance Name: sox-9 transcription factor

Registry Number: 0

Grant and Affiliation Information for BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1.

AFFILIATION: Genetics Institute, Inc., Cambridge, Massachusetts 02140, USA. mmajumdar@genetics.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Cell Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1 Related Publications

• BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1.
• Cdc42 and ARP2/3-independent regulation of filopodia by an integral membrane lipid-phosphatase-related protein.
• Cell- and isoform-specific increases in arginase expression in acute silica-induced pulmonary inflammation.
• Characterization of monocarboxylate transport in human kidney HK-2 cells.
• Clinical dementia rating workshop: the Asian experience.
• Deficiency in angiotensin AT1a receptors prevents diabetes-induced hypertension.
• Development of antibodies and chimeric molecules for cancer immunotherapy.
• Driving and dementia in older adults: Implementation and evaluation of a continuing education project.
• Dual Stretch Responses of mHCN2 Pacemaker Channels: Accelerated Activation, Accelerated Deactivation.
• Education and Alzheimer disease without dementia: support for the cognitive reserve hypothesis.
• Effect of quercetin on the plasma and intracellular concentrations of saquinavir in healthy adults.
• Flavonoids modulate monocarboxylate transporter-1-mediated transport of gamma-hydroxybutyrate in vitro and in vivo.
• Identifying chemical changes in subchondral bone taken from murine knee joints using Raman spectroscopy.
• Improving standards in orthognathic care: the bigger picture (a national and international perspective).
• Interleukin-1beta signals through a c-Jun N-terminal kinase-dependent inducible nitric oxide synthase and nitric oxide production pathway in Sertoli epithelial cells.
• Nocturnal hypertension in mice consuming a high fructose diet.
• Plasma cortisol and progression of dementia in subjects with Alzheimer-type dementia.
• Recombinant human bone morphogenetic protein-2 maintains the articular chondrocyte phenotype in long-term culture.
• Regulation of Product Synthesis in Cell Cultures of Catharanthus roseus. II. Comparison of Production Media1.
• Regulation of Product Synthesis in Cell Cultures of Catharanthus roseus. III. Alkaloid Metabolism in Cultured Leaf Tissue and Primary Callus1.
• Relationship between renal and cardiovascular changes in a murine model of glucose intolerance.
• Repair of articular cartilage defects one year after treatment with recombinant human bone morphogenetic protein-2 (rhBMP-2).
• Retroviral Vectors Encoding a Reverse Transcription-activated Transgene Efficiently Limit Expression of the Gene to Target Cells.
• Role of the transcription activator Ste12p as a repressor of PRY3 expression.
• Rotary spray congealing of a suspension: effect of disk speed and dispersed particle properties.
• Seizures during intracarotid methohexital and amobarbital testing.
• Stress cardiovascular/autonomic interactions in mice.
• The effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on the healing of full-thickness defects of articular cartilage.
• The role of infertility nurses in ovulation induction programmes.
• Urinary oxytocin as a non-invasive biomarker for neurohypophyseal hormone secretion.