Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis.

Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Abstract Text:

Satoshi Fujita,Takashi Abe,Micah J Drummond,Jerson G Cadenas,Hans C Dreyer,Yoshiaki Sato,Elena Volpi,Blake B Rasmussen,Satoshi Fujita,Takashi Abe,Micah J Drummond,Jerson G Cadenas,Hans C Dreyer,Yoshiaki Sato,Elena Volpi,Blake B Rasmussen,

Low-intensity resistance exercise training combined with blood flow restriction (REFR) increases muscle size and strength as much as conventional resistance exercise with high loads. However, the cellular mechanism(s) underlying the hypertrophy and strength gains induced by REFR are unknown. We have recently shown that both the mammalian target of rapamycin (mTOR) signaling pathway and muscle protein synthesis (MPS) were stimulated after an acute bout of high-intensity resistance exercise in humans. Therefore, we hypothesized that an acute bout of REFR would enhance mTOR signaling and stimulate MPS. We measured MPS and phosphorylation status of mTOR-associated signaling proteins in six young male subjects. Subjects were studied once during blood flow restriction (REFR, bilateral leg extension exercise at 20% of 1 repetition maximum while a pressure cuff was placed on the proximal end of both thighs and inflated at 200 mmHg) and a second time using the same exercise protocol but without the pressure cuff [control (Ctrl)]. MPS in the vastus lateralis muscle was measured by using stable isotope techniques, and the phosphorylation status of signaling proteins was determined by immunoblotting. Blood lactate, cortisol, and growth hormone were higher following REFR compared with Ctrl (P < 0.05). Ribosomal S6 kinase 1 (S6K1) phosphorylation, a downstream target of mTOR, increased concurrently with a decreased eukaryotic translation elongation factor 2 (eEF2) phosphorylation and a 46% increase in MPS following REFR (P < 0.05). MPS and S6K1 phosphorylation were unchanged in the Ctrl group postexercise. We conclude that the activation of the mTOR signaling pathway appears to be an important cellular mechanism that may help explain the enhanced muscle protein synthesis during REFR.

Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Publishing Authors By Initials

S Fujita,T Abe,MJ Drummond,JG Cadenas,HC Dreyer,Y Sato,E Volpi,BB Rasmussen,S Fujita,T Abe,MJ Drummond,JG Cadenas,HC Dreyer,Y Sato,E Volpi,BB Rasmussen,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of applied physiology (Bethesda, Md. : 198

VOLUME: 103

Page Numbers: 903-10

Journal Abbreviation: J. Appl. Physiol.

ISSN: 8750-7587

DAY: 14

MONTH: 06

YEAR: 2007

Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8502536

Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis.

AFFILIATION: Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas, USA. fujita@k.u-tokyo.ac.jp

Country: United States

AGENCY: United States NCRR

GRANT: S10-RR-16650

ACRONYM: RR

MEDLINETA: J Appl Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis Related Publications

• A Chronic Increase in Energy Expenditure Inhibits S6K1 and Reduces IRS-1 Serine Phosphorylation in Muscle: 540: May 30 10:30 AM - 10:45 AM.
• Association between intelligence and coronary heart disease mortality: a population-based cohort study of 682 361 Swedish men.
• Association of maternal obesity before conception with poor lactation performance.
• Blood Flow Restriction during Low-Intensity Resistance Exercise Increases Muscle Protein Synthesis through the mTOR Signaling Pathway: 666: May 31 8:00 AM 8:15 AM.
• Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk.
• Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942.
• Cranial implant design using augmented reality immersive system.
• Distinct distribution of autosomal dominant spinocerebellar ataxia in the Mexican population.
• Dysregulation of muscle fatty acid metabolism in type 2 diabetes is independent of malonyl-CoA.
• Experiences with carrier-mediated transport in liquid-phase microextraction.
• Extraction across supported liquid membranes by use of electrical fields.
• Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells.
• Haptic rendering of volumetric data for cranial implant modeling.
• Hysterectomy is associated with stress incontinence in women who previously had a transcervical endometrial resection.
• Impulse control disorders in adults with obsessive compulsive disorder.
• Inhibitory activities of bovine macromolecular whey proteins on rotavirus infections in vitro and in vivo.
• Inhibitory effects of human and bovine milk constituents on rotavirus infections.
• Late-onset obsessive compulsive disorder: clinical characteristics and psychiatric comorbidity.
• Mechanisms controlling death, survival and proliferation in a model unicellular eukaryote Tetrahymena thermophila.
• Microemulsion electrokinetic chromatography in suppressed electroosmotic flow environment. Separation of fat-soluble vitamins.
• Muscle Protein Synthesis is Enhanced Post-Resistance Exercise by Nutrient Stimulation of the mTOR Signaling Pathway: 873: June 1 1:30 PM - 1:45 PM.
• Olanzapine-induced weight gain and increased visceral adiposity is blocked by melatonin replacement therapy in rats.
• Opening rates of DNA hairpins: experiment and model.
• Postoperative cognitive dysfunction in patients with preoperative cognitive impairment: which domains are most vulnerable?
• Sexual obsessions and clinical correlates in adults with obsessive-compulsive disorder.
• Simulation of eye disease in virtual reality.
• Small RNAs controlling outer membrane porins.
• Stress distributions in diblock copolymers.
• Taboo thoughts and doubt/checking: a refinement of the factor structure for obsessive-compulsive disorder symptoms.
• The brown longitudinal obsessive compulsive study: treatments received and patient impressions of improvement.