Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone.

Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone. Research Abstract Details 

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Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone. Abstract Text:

Douglas A Granger,Dante Cicchetti,Fred A Rogosch,Leah C Hibel,Michael Teisl,Elisa Flores,

The prevalence, stability, and impact of blood contamination in children's saliva on the measurement of three of the most commonly assayed hormones were examined. Participants were 363 children (47% boys; ages 6-13 years) from economically disadvantaged families who donated saliva samples on 2 days in the morning, midday, and late afternoon. Samples (n=2178) were later assayed for cortisol (C), testosterone (T), and dehydroepiandrosterone (DHEA). To index the presence of blood (and its components) in saliva, samples were assayed for transferrin. Transferrin levels averaged 0.37 mg/dl (SD=0.46, range 0.0-5.5, Mode=0), and were: (1) highly associated within individuals across hours and days, (2) positively correlated with age, (3) higher for boys than girls, (4) higher in PM than AM samples, and (5) the highest (>1.0 mg/dl) levels were rarely observed in samples donated from the same individuals. Transferrin levels were associated with salivary DHEA and C, but less so for T. As expected, the relationships were positive, and explained only a small portion of the variance. Less than 1% of the statistical outliers (+2.5 SDs) in salivary hormone distributions had correspondingly high transferrin levels. We conclude that blood contamination in children's saliva samples is rare, and its effects on the measurement of salivary hormones is small. Guidelines and recommendations are provided to steer investigators clear of this potential problem in special circumstances and populations.

Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone. Publishing Authors By Initials

DA Granger,D Cicchetti,FA Rogosch,LC Hibel,M Teisl,E Flores,

For similar proteins: blood proteins: acute-phase proteins: transferrin research abstracts see: proteins: blood proteins: acute-phase proteins: transferrin research

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Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Psychoneuroendocrinology

VOLUME: 32

Page Numbers: 724-33

Journal Abbreviation: Psychoneuroendocrinology

ISSN: 0306-4530

DAY: 20

MONTH: 06

YEAR: 2007

Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7612148

Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone. Keywords Mesh Terms:

KEYWORDS: Transferrin

MESH TERMS: analysis

Chemical & Substance for Abstract: Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone. Information

Substance Name: Testosterone

Registry Number: 58-22-0

Grant and Affiliation Information for Blood contamination in children's saliva: prevalence, stability, and impact on the measurement of salivary cortisol, testosterone, and dehydroepiandrosterone.

AFFILIATION: Behavioral Endocrinology Laboratory, Department of Biobehavioral Health, The Pennsylvania State University, 315 Health and Human Development East, University Park, PA 16802, USA. dag11@psu.edu

Country: England

AGENCY: United States NIDA

GRANT: DA 17741

ACRONYM: DA

MEDLINETA: Psychoneuroendocrinology

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