Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow.

Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow. Abstract Text:

Using heparinized whole blood and flow conditions, it was shown that adenosine 5'-diphosphate (ADP) receptors P2Y(12) and P2Y(1) are both important in direct shear-induced platelet aggregation and platelet aggregation subsequent to initial adhesion onto von Willebrand factor (vWf)-collagen. In the viscometer, whole blood was subjected to shear rates of 750, 1500, and 3000 s(-1) for 30 seconds at room temperature. The extent of aggregation was determined by flow cytometry. The P2Y(12) antagonist AR-C69 931MX (ARMX) reduced shear-induced aggregation at these rates by 56%, 54%, and 16%, respectively, compared to control samples. Adenosine 3',5'-diphosphate (A3P5P; P2Y(1) antagonist) inhibited shear-induced aggregation by 40%, 30% and 29%, respectively, compared to control samples. Blockade of both ADP receptors at 3000 s(-1) with ARMX plus A3P5P further reduced the platelet aggregation by 41% compared to the addition of ARMX alone (57% compared to control samples). Using a parallel-plate flow chamber, whole blood was perfused over bovine collagen type 1 at a wall shear rate of 3000 s(-1) for 60 seconds. Platelet deposition was quantified with epifluorescence video microscopy and digital image processing. Blockade of P2Y(12) alone or blockade of P2Y(1) alone did not reduce thrombus formation on vWf-collagen. In contrast, blockade of both P2Y(12) and P2Y(1) reduced platelet deposition by 72%. These results indicate that combinations of antagonists of the ADP receptors P2Y(12) and P2Y(1) are effective inhibitors of direct shear-induced platelet aggregation and of platelet aggregation subsequent to initial adhesion under flow conditions. Inhibitors of these pathways are potentially useful as antiarterial thrombotic agents.

Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Blood

VOLUME: 98

Page Numbers: 3340-5

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 1

MONTH: Dec

YEAR: 2001

Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7603509

Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow. Keywords Mesh Terms:

KEYWORDS: Receptors, Purinergic P2

MESH TERMS: antagonists & inhibitors

Chemical & Substance for Abstract: Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow. Information

Substance Name: Adenosine Monophosphate

Registry Number: 61-19-8

Grant and Affiliation Information for Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow.

AFFILIATION: Department of Bioengineering, Rice University, Houston, TX 77251, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL-54169

ACRONYM: HL

MEDLINETA: Blood

REFSOURCE: Blood. 2002 Jun 15;99(12):4644-5; author

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Blockade of adenosine diphosphate receptors P2Y12 and P2Y1 is required to inhibit platelet aggregation in whole blood under flow Related Publications

• America needs the older handicapped worker.
• Blockade of adenosine diphosphate receptors P2Y(12) and P2Y(1) is required to inhibit platelet aggregation in whole blood under flow.
• Cyclic strain and motion control produce opposite oxidative responses in two human endothelial cell types.
• Enhanced platelet adhesion and aggregation by endothelial cell-derived unusually large multimers of von Willebrand factor.
• For now and the future.
• Gene Expression of Endothelial Cells due to Interleukin-1 Beta Stimulation and Neutrophil Transmigration.
• Gene expression of endothelial cells under pulsatile non-reversing vs. steady shear stress; comparison of nitric oxide production.
• HIV transmission during invasive radiologic procedures: estimate based on computer modeling.
• Hydrodynamic shear stress and mass transport modulation of endothelial cell metabolism.
• Loss of RAB-3/A in Caenorhabditis elegans and the mouse affects behavioral response to ethanol.
• Needle-stick injuries and blood contacts during invasive radiologic procedures: frequency and risk factors.
• Oxidative stress produced with cell migration increases synthetic phenotype of vascular smooth muscle cells.
• Treatment of metal-contaminated water using bacterial sulfate reduction: Results from pilot-scale reactors.