Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system.

Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system. Research Abstract Details 

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Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system. Abstract Text:

Katarzyna Popadiak,Jan Potempa,Kristian Riesbeck,Anna M Blom,

Periodontitis is an inflammatory disease of the supporting structures of the teeth and is caused by, among other agents, Porphyromonas gingivalis. P. gingivalis is very resistant to killing by human complement, which is present in a gingival fluid at 70% of the serum concentration. We found that the incubation of human serum with purified cysteine proteases of P. gingivalis (gingipains) or P. gingivalis wild-type strains W83 and W50 resulted in a drastic decrease of the bactericidal activity of the serum. In contrast, serum treated with P. gingivalis mutants lacking gingipains (particularly strains without HRgpA) maintained significant bactericidal activity. To understand in detail the mechanism by which gingipains destroy the serum bactericidal activity, we investigated the effects of gingipains on the human complement system. We found that all three proteases degraded multiple complement components, with arginine-specific gingipains (HRgpA and RgpB) being more efficient than lysine-specific gingipain (Kgp). Interestingly, all three proteases at certain concentrations were able to activate the C1 complex in serum, which resulted in the deposition of C1q on inert surfaces and on bacteria themselves. It is therefore plausible that P. gingivalis activates complement when present at low numbers, resulting in a local inflammatory reaction and providing the bacteria with a colonization opportunity and nutrients. At later stages of infection the concentration of proteases is high enough to destroy complement factors and thus render the bacteria resistant to the bactericidal activity of complement.

Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system. Publishing Authors By Initials

K Popadiak,J Potempa,K Riesbeck,AM Blom,

For similar proteins: protein subunits research abstracts see: proteins: protein subunits research

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Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 178

Page Numbers: 7242-50

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 1

MONTH: Jun

YEAR: 2007

Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985117

Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system. Keywords Mesh Terms:

KEYWORDS: Protein Subunits

MESH TERMS: antagonists & inhibitors

Chemical & Substance for Abstract: Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system. Information

Substance Name: argingipain, Porphyromonas gingivalis

Registry Number: EC 3.4.22.37

Grant and Affiliation Information for Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system.

AFFILIATION: Lund University, Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmö, Malmö, Sweden.

Country: United States

AGENCY: United States NIDCR

GRANT: DE 09761

ACRONYM: DE

MEDLINETA: J Immunol

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