Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism.

Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism. Research Abstract Details 

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Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism. Abstract Text:

Qing-Quan Chen,Wen-Lan Liu,Xun Guo,Yuan-Jian Li,Zhao-Gui Guo,

AIM: To investigate the effect of aspirin on the apoptosis of cultured bovine aortic endothelial cells (BAEC) and the signal pathways involved in this process. METHODS: BAEC were cultured and passaged in Dulbecco's modified Eagle's medium culture medium. Morphologic changes and quantification of apoptotic cells were determined using fluorescence microscope after staining the cells with Hoechst 33258. Cell viability was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide (MTT) method. DNA fragmentation was visualized by agarose gel electrophoresis. Phospho-p38 mitogen-activated protein kinase (MAPK) expression was detected by Western blotting. RESULTS: Aspirin at low concentrations from 1X10( -10) mol/L to 1X10( -8) mol/L decreased the apoptosis and p38 MAPK phosphorylation induced by H2O2 in BAEC, while high doses of aspirin (1X10( -7)-1X10( -4) mol/L) induced typical apoptotic changes in BAEC and stimulated the expression of phospho-p38 MAPK in a concentration-dependent manner. SB203580, a specific p38 MAPK inhibitor, blocked such effects. CONCLUSION: Aspirin exhibits a biphasic effect on the apoptosis in BAEC, reducing apoptosis at low concentration and inducing apoptosis at high concentration. p38 MAPK may be an important signal molecule mediating the effects of aspirin.

Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism. Publishing Authors By Initials

QQ Chen,WL Liu,X Guo,YJ Li,ZG Guo,

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Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta pharmacologica Sinica

VOLUME: 28

Page Numbers: 353-8

Journal Abbreviation: Acta Pharmacol. Sin.

ISSN: 1671-4083

DAY: 16

MONTH: Mar

YEAR: 2007

Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism. Information

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LANGUAGE: eng

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AFFILIATION: Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China.

Country: China

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MEDLINETA: Acta Pharmacol Sin

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