Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports.

Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports. Research Abstract Details 

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Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports. Abstract Text:

Rachel G Handwerger,Scott L Diamond,

A biotinylated photocleavable polyethylenimine (B-PC-PEI) was designed and synthesized for the capture and controlled release of nucleic acids from solid supports. B-PC-PEI was synthesized via a three-step reaction process and verified by 1H NMR and mass spectrometry. In aqueous solution, the o-nitrobenzyl group within B-PC-PEI was efficiently cleaved by 5 min of 365 nm light exposure from a distance of 20 cm (9 mW/cm2). When coupled to streptavidin-coated beads, the PEI domain of Cy5-labeled B-PC-PEI was released by 365 nm light exposure. In contrast, a Cy5-labeled biotinylated PEI (B-PEI) was used as a control and negligible fluorescence loss was observed. Cy5-labeled siRNA was electrostatically captured to streptavidin-coated beads preabsorbed with B-PC-PEI or B-PEI, and flow cytometry demonstrated significant loss of fluorescence from the bead surface after 5 min of light exposure only for B-PC-PEI, demonstrating controlled release of siRNA from the bead surface. Finally, the release of the Cy5-labeled siRNA into the supernatant was quantified. The release of Cy5-siRNA into the supernatant was significantly greater after 5 min of light exposure for B-PC-PEI/streptavidin beads compared to 0 min exposure and remained unchanged for B-PEI/streptavidin beads. B-PC-PEI facilitates capture and triggered release of surface-tethered nucleic acids with light exposure and is fully compatible with streptavidin-based applications.

Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports. Publishing Authors By Initials

RG Handwerger,SL Diamond,

For similar proteins: bacterial proteins: streptavidin research abstracts see: proteins: bacterial proteins: streptavidin research

PUBMED ID PMID:

MEDLINE DATE: 2007 May-Jun

Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Bioconjugate chemistry

VOLUME: 18

Page Numbers: 717-23

Journal Abbreviation: Bioconjug. Chem.

ISSN: 1043-1802

DAY: 14

MONTH: 04

YEAR: 2007

Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9010319

Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports. Keywords Mesh Terms:

KEYWORDS: Streptavidin

MESH TERMS: chemistry

Chemical & Substance for Abstract: Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports. Information

Substance Name: Streptavidin

Registry Number: 9013-20-1

Grant and Affiliation Information for Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports.

AFFILIATION: Institute for Medicine and Engineering, 1024 Vagelos Laboratories, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL66565

ACRONYM: HL

MEDLINETA: Bioconjug Chem

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