Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression.

Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression. Research Abstract Details 

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Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression. Abstract Text:

May P Xiong,M Laird Forrest,Angela L Karls,Glen S Kwon,

Covalently poly(ethylene glycol) (PEG)-ylated polyethylenimine (PEI)/pDNA complexes display prolonged blood circulation profiles compared with PEI/pDNA complexes, but such PEGylated particles may not be suitable for tumor targeting due to low interaction with cell membranes, low internalization, and low gene expression. Noncovalent PEGylation of cationic particles via PEG-avidin/biotin-PEI is an attempt to bridge the gap between the positive attributes of PEG (prolonged particle circulation) and the positive attributes of nontoxic cationic polymers (enhanced cell interactions) for greater gene expression. Our polymer, 2PEG-avidin/biotin-PEI8, forms salt-stable particles ( approximately 100 nm) under physiologic conditions with a minimum of two 2PEG-avidin molecules bound per polymer chain (biotin-PEI8, 8 biotins/PEI). Following 10 days of incubation with 3000-fold excess biotin, 2PEG-avidin completely dissociated from biotin-PEI8, and gene expression was increased 2.1-32-fold in various cell lines when the desirable transfection feature of the cationic polymer was retained. This new PEGylation approach has implications for generally improving the clinical aspect of gene delivery via a two-step therapeutic strategy: (1) intravenous injection of noncovalent PEG-avidin/biotin-polycation nanoparticles for prolonged circulation, followed by (2) temporal release of PEG-avidin from biotin-polycation through either endogenous biotin or intravenous injection of biotin.

Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression. Publishing Authors By Initials

MP Xiong,ML Forrest,AL Karls,GS Kwon,

For similar organic chemicals: hydrocarbons: hydrocarbons, acyclic: alkenes: polyenes: polyethylenes: polyethyleneimine research abstracts see: organic chemicals: hydrocarbons: hydrocarbons, acyclic: alkenes: polyenes: polyethylenes: polyethyleneimine research

PUBMED ID PMID:

MEDLINE DATE: 2007 May-Jun

Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Bioconjugate chemistry

VOLUME: 18

Page Numbers: 746-53

Journal Abbreviation: Bioconjug. Chem.

ISSN: 1043-1802

DAY: 22

MONTH: 03

YEAR: 2007

Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9010319

Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression. Keywords Mesh Terms:

KEYWORDS: Polyethyleneimine

MESH TERMS: chemistry

Chemical & Substance for Abstract: Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression. Information

Substance Name: Polyethyleneimine

Registry Number: 9002-98-6

Grant and Affiliation Information for Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression.

AFFILIATION: Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin, 777 Highland Avenue, Madison, Wisconsin 53705-2222, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI-43346-08

ACRONYM: AI

MEDLINETA: Bioconjug Chem

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