Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin.

Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin. Abstract Text:

K M Tytgat,H A ,F J Opdam,Y S Kim,A W Einerhand,J Dekker,

BACKGROUND/AIMS: Human colonic epithelium produces large amounts of mucin. The aim of this study was to examine mucin biosynthesis in the human colon. METHODS: Human colonic mucin was isolated using CsCl density gradients, and polyclonal antiserum was raised. Biosynthesis of colonic mucins was studied by labeling colonic explants with 35S-labeled amino acids or [35S]sulfate and subsequent immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: The polyclonal antiserum specifically recognized colonic mucin, primarily reacting with peptide epitopes. Biosynthetic pulse/chase experiments showed a 35S-amino acid-labeled mucin precursor of about 600 kilodaltons, which was converted into a mature, glycosylated, and sulfated mucin and subsequently secreted into the medium. This mature mucin comigrated with isolated colonic mucin with an apparent molecular weight of 550 kilodaltons on SDS-PAGE, whereas gel filtration indicated that the molecular weight is actually much larger. Independent immunoprecipitation with an anti-Muc2 antiserum showed cross-reactivity with the 600-kilodalton precursor. CONCLUSIONS: These results show the biosynthesis of a secretory colonic mucin for the first time. This mucin is synthesized as a precursor protein of approximately 600 kilodaltons, which, after glycosylation, is secreted as a glycoprotein with an apparent molecular weight of 550 kilodaltons on SDS-PAGE. It is very likely that this mucin is Muc2.

Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin. Publishing Authors By Initials

KM Tytgat,HA ,FJ Opdam,YS Kim,AW Einerhand,J Dekker,

For similar proteins: protein precursors research abstracts see: proteins: protein precursors research

PUBMED ID PMID:

MEDLINE DATE:

Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Gastroenterology

VOLUME: 107

Page Numbers: 1352-63

Journal Abbreviation: Gastroenterology

ISSN: 0016-5085

DAY: 30

MONTH: Nov

YEAR: 1994

Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374630

Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin. Keywords Mesh Terms:

KEYWORDS: Protein Precursors

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin. Information

Substance Name: Protein Precursors

Registry Number: 0

Grant and Affiliation Information for Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin.

AFFILIATION: Department of Pediatric Gastroenterology and Nutrition, Academic Medical Center, Amsterdam, The Netherlands.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Gastroenterology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin Related Publications

• A meta-analysis quantifying transmission parameters of FMDV strain O Taiwan among non-vaccinated and vaccinated pigs.
• An RNA toolbox for single-molecule force spectroscopy studies.
• Barrett's esophagus is characterized by expression of gastric-type mucins (MUC5AC, MUC6) and TFF peptides (TFF1 and TFF2), but the risk of carcinoma development may be indicated by the intestinal-type mucin, MUC2.
• Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin.
• Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3 and more.
• Biosynthesis of rat MUC2 in colon and its analogy with human MUC2.
• Cognitive Behavioral Therapy versus Short Psychodynamic Supportive Psychotherapy in the outpatient treatment of depression: a randomized controlled trial.
• Confounding: What it is and how to deal with it.
• Criminalization of medical error: who draws the line?
• Does early response predict outcome in psychotherapy and combined therapy for major depression?
• Effect of intrauterine growth restriction on kidney function at young adult age: the Nord Trøndelag Health (HUNT 2) Study.
• Fate of goblet cells in experimental colitis.
• GC- and AT-rich chromatin domains differ in conformation and histone modification status and are differentially modulated by Rpd3p.
• Gastric-type mucin and TFF-peptide expression in Barrett's oesophagus is disturbed during increased expression of MUC2.
• Gastrointestinal expression and partial cDNA cloning of murine Muc2.
• High plasma phosphate as a risk factor for decline in renal function and mortality in pre-dialysis patients.
• Interleukin 10-deficient mice exhibit defective colonic Muc2 synthesis before and after induction of colitis by commensal bacteria.
• Long-term effectiveness of exercise therapy in patients with osteoarthritis of the hip or knee: a systematic review.
• Mapping networks of physical interactions between genomic elements using 5C technology.
• Metaplasia of the duodenum shows a Helicobacter pylori-correlated differentiation into gastric-type protein expression.
• Muc2 in ulcerative colitis: a quantitative study.
• Persistence length measurements from stochastic single-microtubule trajectories.
• Reliability studies can be designed more efficiently by using variance components estimates from different sources.
• Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats.
• Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis.
• The nature of the excited state of the reaction center of photosystem II of green plants: A high-resolution fluorescence spectroscopy study.
• The oligomerization of a family of four genetically clustered human gastrointestinal mucins.
• The valuable contribution of observational studies to nephrology.
• Three-dimensional photoacoustic imaging of blood vessels in tissue.
• [Fixation of syndesmotic disruption using bioresorbable screws.]