Biosynthesis and turnover of carnitine acetyltransferase in rat liver.

Biosynthesis and turnover of carnitine acetyltransferase in rat liver. Research Abstract Details 

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Biosynthesis and turnover of carnitine acetyltransferase in rat liver. Abstract Text:

S Miyazawa,H Ozasa,S Furuta,T Osumi,T Hashimoto,S Miura,M Mori,M Tatibana,

Male Wistar rats were fed on a diet with and without di(2-ethylhexyl)phthalate (DEHP) for 2 weeks. Carnitine acetyltransferase in the liver was increased about 100-fold by administration of DEHP. The results of in vivo experiments showed that the incorporation of L-[4,5-3H]leucine into the enzyme was 12-fold higher and the half-life of the labeled enzyme was elongated by a factor 4.6. The results of in vitro translation experiments with total hepatic RNA in a rabbit reticulocytelysate system and the results concerning the synthesis of the enzyme in isolated hepatocytes indicate that the translatable mRNA for the enzyme was increased upon administration of DEHP and that the enzyme is synthesized as a precursor (Mw = 69,000) larger than the mature enzyme (Mw = 67,500). RNA in the free polysomes directed the synthesis of the enzyme precursor five times more actively than RNA in membrane-bound polysomes.

Biosynthesis and turnover of carnitine acetyltransferase in rat liver. Publishing Authors By Initials

S Miyazawa,H Ozasa,S Furuta,T Osumi,T Hashimoto,S Miura,M Mori,M Tatibana,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains research

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Biosynthesis and turnover of carnitine acetyltransferase in rat liver. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 93

Page Numbers: 453-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1983

Biosynthesis and turnover of carnitine acetyltransferase in rat liver. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Biosynthesis and turnover of carnitine acetyltransferase in rat liver. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred Strains

MESH TERMS: genetics

Chemical & Substance for Abstract: Biosynthesis and turnover of carnitine acetyltransferase in rat liver. Information

Substance Name: Carnitine O-Acetyltransferase

Registry Number: EC 2.3.1.7

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Country: JAPAN

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MEDLINETA: J Biochem

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