Biomarkers to disclose recent intake of alcohol: potential of 5-hydroxytryptophol glucuronide testing using new direct UPLC-tandem MS and ELISA methods.

Biomarkers to disclose recent intake of alcohol: potential of 5-hydroxytryptophol glucuronide testing using new direct UPLC-tandem MS and ELISA methods. Research Abstract Details 

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Biomarkers to disclose recent intake of alcohol: potential of 5-hydroxytryptophol glucuronide testing using new direct UPLC-tandem MS and ELISA methods. Abstract Text:

Olof Beck,Nikolai Stephanson,Michael ,Norbert Dahmen,Christoph Fehr,Anders Helander,Olof Beck,Nikolai Stephanson,Michael ,Norbert Dahmen,Christoph Fehr,Anders Helander,Olof Beck,Nikolai Stephanson,Michael ,Norbert Dahmen,Christoph Fehr,Anders Helander,

AIMS: This study compared two new methods for direct determination of 5-hydroxytryptophol glucuronide (GTOL) in urine, a biomarker for detection of recent alcohol consumption. METHODS: Urine samples were collected from ten alcoholic patients during recovery from intoxication. A direct injection ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for measurement of the urinary GTOL to 5-hydroxyindoleacetic acid (5-HIAA) ratio, and an ELISA assay for direct measurement of GTOL, were used. Comparison was made with the urinary ethanol and ethyl glucuronide (EtG) concentrations. RESULTS: The breath ethanol concentration on admission ranged between 1.0-3.1 g/l. The UPLC-MS/MS method showed a median detection time of 39 h for an elevated urinary GTOL/5-HIAA ratio, while EtG was detected for a median of 65 h. Determination of GTOL by the ELISA assay showed 87% sensitivity in detecting positive samples at a 44% specificity, as compared with the UPLC-MS/MS method. CONCLUSIONS: The lower sensitivity of the urinary GTOL/5-HIAA ratio compared with EtG for recent drinking may be clinically useful, in cases where the EtG test provides an unwanted high sensitivity for intake of only small amounts of alcohol or unintentional ethanol exposure.

Biomarkers to disclose recent intake of alcohol: potential of 5-hydroxytryptophol glucuronide testing using new direct UPLC-tandem MS and ELISA methods. Publishing Authors By Initials

O Beck,N Stephanson,M ,N Dahmen,C Fehr,A Helander,O Beck,N Stephanson,M ,N Dahmen,C Fehr,A Helander,O Beck,N Stephanson,M ,N Dahmen,C Fehr,A Helander,

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PUBMED ID PMID:

MEDLINE DATE: 2007 Jul-Aug

Biomarkers to disclose recent intake of alcohol: potential of 5-hydroxytryptophol glucuronide testing using new direct UPLC-tandem MS and ELISA methods. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Alcohol and alcoholism (Oxford, Oxfordshire)

VOLUME: 42

Page Numbers: 321-5

Journal Abbreviation: Alcohol Alcohol.

ISSN: 0735-0414

DAY: 28

MONTH: 05

YEAR: 2007

Biomarkers to disclose recent intake of alcohol: potential of 5-hydroxytryptophol glucuronide testing using new direct UPLC-tandem MS and ELISA methods. Information

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LANGUAGE: eng

NlmUniqueID: 8310684

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AFFILIATION: Department of Medicine, Karolinska Institute and Karolinska University Hospital, Stockholm, Sweden. olof.beck@karolinska.se

Country: England

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MEDLINETA: Alcohol Alcohol

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