Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds.

Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds. Abstract Text:

Nanofibrous materials, by virtue of their morphological similarities to natural extracellular matrix, have been considered as candidate scaffolds for cell delivery in tissue-engineering applications. In this study, we have evaluated a novel, three-dimensional, nanofibrous poly(epsilon-caprolactone) (PCL) scaffold composed of electrospun nanofibers for its ability to maintain chondrocytes in a mature functional state. Fetal bovine chondrocytes (FBCs), maintained in vitro between passages 2 to 6, were seeded onto three-dimensional biodegradable PCL nanofibrous scaffolds or as monolayers on standard tissue culture polystyrene (TCPS) as a control substrate. Gene expression analysis by reverse transcription-polymerase chain reaction showed that chondrocytes seeded on the nanofibrous scaffold and maintained in serum-free medium supplemented with ITS+, ascorbate, and dexamethasone continuously maintained their chondrocytic phenotype by expressing cartilage-specific extracellular matrix genes, including collagen types II and IX, aggrecan, and cartilage oligomeric matrix protein. Specifically, expression of the collagen type IIB splice variant transcript, which is indicative of the mature chondrocyte phenotype, was up-regulated. FBCs exhibited either a spindle or round shape on the nanofibrous scaffolds, in contrast to a flat, well-spread morphology seen in monolayer cultures on TCPS. Organized actin stress fibers were only observed in the cytoplasm of cells cultured on TCPS. Histologically, nanofibrous cultures maintained in the supplemented serum-free medium produced more sulfated proteoglycan-rich, cartilaginous matrix than monolayer cultures. In addition to promoting phenotypic differentiation, the nanofibrous scaffold also supported cellular proliferation as evidenced by a 21-fold increase in cell growth over 21 days when the cultures were maintained in serum-containing medium. These results indicate that the biological activities of FBCs are crucially dependent on the architecture of the extracellular scaffolds as well as the composition of the culture medium, and that nanofibrous PCL acts as a biologically preferred scaffold/substrate for proliferation and maintenance of the chondrocytic phenotype. We propose that the PCL nanofibrous structure may be a suitable candidate scaffold for cartilage tissue engineering.

Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical materials research. Part A

VOLUME: 67

Page Numbers: 1105-14

Journal Abbreviation:

ISSN: 1549-3296

DAY: 15

MONTH: Dec

YEAR: 2003

Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101234237

Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds. Keywords Mesh Terms:

KEYWORDS: Polyesters

MESH TERMS: metabolism

Chemical & Substance for Abstract: Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds. Information

Substance Name: polycaprolactone

Registry Number: 24980-41-4

Grant and Affiliation Information for Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds.

AFFILIATION: Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Services, Building 50, Room 1503, MSC8022, Bethesda, Maryland 20892-8022, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biomed Mater Res A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Biological response of chondrocytes cultured in three-dimensional nanofibrous polyepsilon-caprolactone scaffolds Related Publications

• A facilitated tracking and transcription mechanism of long-range enhancer function.
• A second-generation autologous chondrocyte implantation approach to the treatment of focal articular cartilage defects.
• Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts.
• Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds.
• Cell density dependent regulation of AP-1 activity is important for chondrogenic differentiation of C3H10T1/2 mesenchymal cells.
• Cellular signaling in developmental chondrogenesis: N-cadherin, Wnts, and BMP-2.
• Chondrogenic differentiation of murine C3H10T1/2 multipotential mesenchymal cells: I. Stimulation by bone morphogenetic protein-2 in high-density micromass cultures.
• Chondrogenic differentiation of murine C3H10T1/2 multipotential mesenchymal cells: II. Stimulation by bone morphogenetic protein-2 requires modulation of N-cadherin expression and function.
• Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.
• Depression status as a confounder in the effects of antidepressants on the gestation age.
• Fabrication and characterization of six electrospun poly(alpha-hydroxy ester)-based fibrous scaffolds for tissue engineering applications.
• Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells.
• Investigation of enablers of knowledge transfer in the medical industry.
• Mechanism of BMP-2 stimulated adhesion of osteoblastic cells to titanium alloy.
• Memory encoded throughout our bodies: molecular and cellular basis of tissue regeneration.
• Mesenchymal stem cell-based cartilage tissue engineering: cells, scaffold and biology.
• Mold-shaped, nanofiber scaffold-based cartilage engineering using human mesenchymal stem cells and bioreactor.
• Murine C3H10T1/2 multipotential cells as an in vitro model of mesenchymal chondrogenesis.
• Regional multilineage differentiation potential of meniscal fibrochondrocytes: implications for meniscus repair.
• Regulation of cartilaginous ECM gene transcription by chondrocytes and MSCs in 3D culture in response to dynamic loading.
• Repair of porcine articular cartilage defect with a biphasic osteochondral composite.
• Simulated joint infection assessment by rapid detection of live bacteria with real-time reverse transcription polymerase chain reaction.
• Spatiotemporal protein distribution of TGF-betas, their receptors, and extracellular matrix molecules during embryonic tendon development.
• TGF-beta1 calcium signaling in osteoblasts.
• Testing and estimation for variable single evoked brain potentials.
• Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction.
• Wnt signaling during BMP-2 stimulation of mesenchymal chondrogenesis.
• Wnt-3A enhances bone morphogenetic protein-2-mediated chondrogenesis of murine C3H10T1/2 mesenchymal cells.
• [30 Cases of pulmonary atelectasia during the course of acute broncho-pneumophathies in children.]
• [Primary cancer of the liver in a child 14 months old.]