Bioconjugated nanoparticles for DNA protection from cleavage.

Bioconjugated nanoparticles for DNA protection from cleavage. Research Abstract Details 

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Bioconjugated nanoparticles for DNA protection from cleavage. Abstract Text:

Xiao-xiao He,Kemin Wang,Weihong Tan,Bin Liu,Xia Lin,Chunmei He,Du Li,Shasheng Huang,Jun Li,

We have developed a novel method to protect DNA from cleavage using bioconjugated nanoparticles. Positively charged amino-modified silica nanoparticles have been directly prepared using water-in-oil microemulsion. Plasmid DNA can be easily enriched onto the positively charged nanoparticle surface, and the DNA strands are well protected from enzymatic cleavage. When incubated with nuclease enzyme for enzymatic cleavage, free plasmid DNA strands are completely cleaved, while those on the nanoparticle surfaces are intact. Our results clearly demonstrate unique properties of nanomaterials when combined with biomolecules. Our simple bionanotechnology will be highly useful in DNA separation, manipulation, and detection, and possibly in genetic engineering and gene therapy, as plasmid DNA can be protected in cellular environments without any change in its property.

Bioconjugated nanoparticles for DNA protection from cleavage. Publishing Authors By Initials

XX He,K Wang,W Tan,B Liu,X Lin,C He,D Li,S Huang,J Li,

For similar inorganic chemicals: minerals: silicon dioxide research abstracts see: inorganic chemicals: minerals: silicon dioxide research

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Bioconjugated nanoparticles for DNA protection from cleavage. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 7168-9

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 18

MONTH: Jun

YEAR: 2003

Bioconjugated nanoparticles for DNA protection from cleavage. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Bioconjugated nanoparticles for DNA protection from cleavage. Keywords Mesh Terms:

KEYWORDS: Silicon Dioxide

MESH TERMS: chemistry

Chemical & Substance for Abstract: Bioconjugated nanoparticles for DNA protection from cleavage. Information

Substance Name: Deoxyribonuclease I

Registry Number: EC 3.1.21.1

Grant and Affiliation Information for Bioconjugated nanoparticles for DNA protection from cleavage.

AFFILIATION: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering, Institute of Biological Technology, Hunan University, Changsha 410082, P. R. China.

Country: United States

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MEDLINETA: J Am Chem Soc

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