Biochemical relationship among three F-type pyocins, pyocin F1, F2, and F3, and phage KF1.

Biochemical relationship among three F-type pyocins, pyocin F1, F2, and F3, and phage KF1. Research Abstract Details 

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Biochemical relationship among three F-type pyocins, pyocin F1, F2, and F3, and phage KF1. Abstract Text:

K Kuroda,R Kagiyama,

The immunological and the biochemical relationships between F-type pyocins and phage KF1, which is cross-reactive with anti-F-type pyocin sera, were studied. The primary structures of six subunit proteins of each pyocin were also compared among three F-type pyocins, pyocin F1, F2, and F3. Both anti-pyocin F1 and anti-pyocin F3 sera gave precipitin bands with phage KF1 by Ouchterlony's test, and were found to bind to minor subunit proteins P3 and P6 of the phage, which were separated by SDS-polyacrylamide gel electrophoresis. Electron microscopic studies showed that these sera bound to some loci of the rod part of the phage tail. These results showed that the subunit proteins P3 and P6 carried antigenically common parts to F-type pyocin and that P3 and/or P6 were components of the rod part of the phage KF1. The subunit proteins P3 and P6 of the phage were of the same mobilities in SDS-polyacrylamide gel electrophoresis as those of band 1 and band 5 of F-type pyocins, respectively. Tryptic peptide mapping after iodination with 125I also showed a partial homology between P3 and band 1, and between P6 and band 5. The tryptic peptide mapping of the six subunit proteins of each F-type pyocin showed that the primary structure of subunit protein band 1 was almost the same among these pyocins, and subunit protein bands 2, 3, 5, and 6 showed were similar. Only subunit protein band 4 was different among these pyocins. The difference in the action spectra of pyocin F1, F2, and F3 is probably due to the difference in the primary structure of subunit protein band 4, which is a component of the fiber part.

Biochemical relationship among three F-type pyocins, pyocin F1, F2, and F3, and phage KF1. Publishing Authors By Initials

K Kuroda,R Kagiyama,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Biochemical relationship among three F-type pyocins, pyocin F1, F2, and F3, and phage KF1. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 1429-41

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1983

Biochemical relationship among three F-type pyocins, pyocin F1, F2, and F3, and phage KF1. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Biochemical relationship among three F-type pyocins, pyocin F1, F2, and F3, and phage KF1. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Biochemical relationship among three F-type pyocins, pyocin F1, F2, and F3, and phage KF1. Information

Substance Name: Viral Proteins

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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