Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem.

Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem. Abstract Text:

S I Hasegawa,S Kaneko,M Okuda,S Araki,M Satake,

Nerve cell bodies, large and multipolar, were isolated in bulk with the least possible contamination from the pig brain stem. The activities of two neurobiologically important membrane enzymes, Na+, K+-ATPase, and acetylcholinesterase, in the isolated cell bodies were estimated. Na+, K+-ATPase [EC 3.6.1.4], more accurately called ouabain-sensitive ATPase of the nerve cell body, hydrolyzed 94 micronmoles of ATP per h per 100 mg of protein. This activity was one-fourth that in the brain stem. Nerve cell bodies contained a large amount of Ca2+, 275 micronmoles per 100 mg of protein, about half of which was calculated to exist as compounds other than calcium orthophosphate. However, the Na+, K+-ATPase of the nerve cell bodies was not stimulated by EGTA, in contrast to that of the brain stem. Acetylcholinesterase [EC 3.1.1.7] and cholinesterase [EC 3.1.1.8] activities were estimated separately by the use of the specific inhibitors Persidol and BW 284C51 dibromide. Acetylcholinesterase was almost completely responsible for the hydrolysis of acetylcholine in the nerve cell bodies isolated from the brain stem and little cholinesterase activity was detected. 1300-1400 micronmoles of acetylcholine was hydrolyzed per h per 100 mg of protein of the neuronal cell bodies; this activity was about four times higher than that in the brain stem. The differences between the specific activities of Na+, K+-ATPase, and acetylcholinesterase in theneuronal cell bodies and the brain stem are discussed in the light of electron microscopic analysis of the distribution of these enzymes and the preservation of the plasma membrane of the isolated cell bodies.

Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem. Publishing Authors By Initials

SI Hasegawa,S Kaneko,M Okuda,S Araki,M Satake,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

PUBMED ID PMID:

MEDLINE DATE:

Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 729-37

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1977

Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem. Information

Substance Name: Adenosine Triphosphatases

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Biochemical characterization of the neuron ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem Related Publications

• 4-O-Acetyl mycarose. A new O-acetyl sugar obtained from leucomycin minor components.
• A SMAP Gene Family Encoding ARF GTPase-Activating Proteins and Its Implication in Membrane Trafficking.
• A novel sphingophosphonoglycolipid containing 3-O-methylgalactose isolated from the skin of the marine gastropod Aplysia kurodai.
• ACID AND ALKALINE DEGRADATION OF THE TNP-AMINO ACIDS AND -PEPTIDES.
• An enzyme cycling method for measurement of allantoin in human serum.
• Biochemical characterization of the neuron. ATPase and acetylcholinesterase activities of neuronal cell bodies isolated in bulk from the pig brain stem.
• Characterization of phosphonoglycosphingolipids containing pyruvate: localization in Aplysia nerve bundles.
• Chemical Constituents of Morinda citrifolia Roots Exhibit Hypoglycemic Effects in Streptozotocin-Induced Diabetic Mice.
• Clathrin dependent endocytosis of E-cadherin is regulated by the Arf6GAP isoform SMAP1.
• Clinical management of autoimmune pancreatitis.
• Comparison of 30 immunity-related genes from the common marmoset with orthologues from human and mouse.
• Different stability of N- and C-domain of diferric ovotransferrin in urea and application to the determination of iron distribution between the two domains.
• Fluorescence Response of a 4-Trifluoroacetylaminophthalimide to Iodide Ions upon 254 nm Irradiation in MeCN.
• Functions of a GnRH receptor heterodimer of the ascidian, Ciona intestinalis. Short communication.
• Gangliosides in neuron.
• Glycolipids in the nervous tissue of invertebrate.
• HEMOGLOBINS FROM ERYTHROCYTES OF EEL, ANGUILLA JAPONICA.
• Heterogeneity of hemoglobin from Arca (Anadara satowi). Molecular weights and oxygen equilibria of Arca Hb I and II.
• Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai.
• Isolation and characterization of a novel 2-aminoethylphosphonyl-glycosphingolipid from the sea hare, Aplysia kurodai.
• Negative cooperativity of chicken ovotransferrin on Al(III)-binding.
• On the chemical structure of porcine globin alpha.
• On the molecular weight of hemoglobin from Anadara inflata at various pH.
• Selective modification of myosin SH1 with 1,2,4-trinitrobenzene. VIII. Thiols of myosin.
• Steady state characteristics of acclimated hydrogenotrophic methanogens on inorganic substrate in continuous chemostat reactors.
• Structural analysis of a novel triphosphonoglycosphingolipid from the egg of the sea hare, Aplysia kurodai.
• Studies on insulin. IV. On the amino acid composition of the glycyl chain of Bonito insulin-I.
• The spectrophotometric determination as trinitrophenyl derivatives of amino acids and peptides resolved on paper by electrophoresis and chromatography.
• [Calcium oxalate crystals in several kinds of Cinnamon bark]
• [Patterns of mediastinal and supraclavicular metastases in apical invasive lung cancer--importance of supraclavicular lymph node dissection]