Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene.

Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene. Research Abstract Details 

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Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene. Abstract Text:

L Q Qu,X L Wei,H Satoh,T Kumamaru,M Ogawa,F Takaiwa,

The rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased (alpha-1H/alpha-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/alpha-2 encoded by glu4 while forming a new polypeptide of pI6.50/alpha-1. Experiments were conducted to identify the relationships between the mutated polypeptides of the mutant and to illustrate the mutation mechanism of the allele. Peptide mapping and amino-acid sequence analyses revealed that the newly formed glu4a encoded polypeptide pI6.50/alpha-1 of high homology with the deleted pI6.71/alpha-2 polypeptide which was encoded by glu4 (GluA-1). The nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable region of the gene. These results suggested the possibility of altering glutelin quality by using single gene mutation.

Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene. Publishing Authors By Initials

LQ Qu,XL Wei,H Satoh,T Kumamaru,M Ogawa,F Takaiwa,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, nucleic acid research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, nucleic acid research

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Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 107

Page Numbers: 20-5

Journal Abbreviation:

ISSN: 0040-5752

DAY: 21

MONTH: 03

YEAR: 2003

Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene. Information

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LANGUAGE: eng

NlmUniqueID: 145600

Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene. Keywords Mesh Terms:

KEYWORDS: Sequence Homology, Nucleic Acid

MESH TERMS: metabolism

Chemical & Substance for Abstract: Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene. Information

Substance Name: Gluten

Registry Number: 8002-80-0

Grant and Affiliation Information for Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene.

AFFILIATION: Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China. lqu@affrc.go.jp

Country: Germany

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MEDLINETA: Theor Appl Genet

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