Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway.

Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Research Abstract Details 

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Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Abstract Text:

K Tyson,C Elkins,H Patterson,E Fikrig,A de Silva,

Ixodes ticks are vectors of several pathogens including Borrelia burgdorferi. Tick saliva contains numerous molecules that facilitate blood feeding without host immune recognition and rejection. We have expressed, purified, and characterized Ixodes scapularis salivary protein 20 (Salp20), a potential inhibitor of the alternative complement pathway that shares homology with the Isac protein family. When analysed by SDS-PAGE and size exclusion chromatography, Salp20 was approximately 48 kDa, more than double its predicted mass, primarily due N- and O-linked glycosylations. Recombinant Salp20 inhibited the alternative complement pathway by dissociating the C3 convertase, and partially protected a serum sensitive species of Borrelia from lysis by normal human serum. We propose that Salp20 facilitates tick feeding and possibly protects tick-borne pathogens from complement components.

Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Publishing Authors By Initials

K Tyson,C Elkins,H Patterson,E Fikrig,A de Silva,

For similar proteins: salivary proteins research abstracts see: proteins: salivary proteins research

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Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Insect molecular biology

VOLUME: 16

Page Numbers: 469-79

Journal Abbreviation: Insect Mol. Biol.

ISSN: 0962-1075

DAY: 3

MONTH: Aug

YEAR: 2007

Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9303579

Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Keywords Mesh Terms:

KEYWORDS: Salivary Proteins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway. Information

Substance Name: Salivary Proteins

Registry Number: 0

Grant and Affiliation Information for Biochemical and functional characterization of Salp20, an Ixodes scapularis tick salivary protein that inhibits the complement pathway.

AFFILIATION: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, NC 27599, USA.

Country: England

AGENCY: United States NIAID

GRANT: 5U01AI058263-04

ACRONYM: AI

MEDLINETA: Insect Mol Biol

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