Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli.

Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli. Research Abstract Details 

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Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli. Abstract Text:

Ho Young Lee,Chaitan Khosla,

Macrolide antibiotics such as erythromycin are clinically important polyketide natural products. We have engineered a recombinant strain of Escherichia coli that produces small but measurable quantities of the bioactive macrolide 6-deoxyerythromycin D. Bioassay-guided evolution of this strain led to the identification of an antibiotic-overproducing mutation in the mycarose biosynthesis and transfer pathway that was detectable via a colony-based screening assay. This high-throughput assay was then used to evolve second-generation mutants capable of enhanced precursor-directed biosynthesis of macrolide antibiotics. The availability of a screen for macrolide biosynthesis in E. coli offers a fundamentally new approach in dissecting modular megasynthase mechanisms as well as engineering antibiotics with novel pharmacological properties.

Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli. Publishing Authors By Initials

HY Lee,C Khosla,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleotides: thymine nucleotides research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleotides: thymine nucleotides research

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Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: PLoS biology

VOLUME: 5

Page Numbers: e45

Journal Abbreviation: PLoS Biol.

ISSN: 1545-7885

DAY: 3

MONTH: Feb

YEAR: 2007

Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101183755

Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Thymine Nucleotides

MESH TERMS: metabolism

Chemical & Substance for Abstract: Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli. Information

Substance Name: mycarose

Registry Number: 6032-92-4

Grant and Affiliation Information for Bioassay-guided evolution of glycosylated macrolide antibiotics in Escherichia coli.

AFFILIATION: Department of Chemistry, Stanford University, Stanford, California, United States of America.

Country: United States

AGENCY: United States NCI

GRANT: CA 66736

ACRONYM: CA

MEDLINETA: PLoS Biol

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