Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies.

Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Abstract Text:

Lakeshia J Taite,Maude L Rowland,Katie A Ruffino,Bryan R E Smith,Michael B Lawrence,Jennifer L West,

The binding of activated integrins on the surface of leukocytes facilitates the adhesion of leukocytes to vascular endothelium during inflammation. Interactions between selectins and their ligands mediate rolling, and are believed to play an important role in leukocyte adhesion, though the minimal recognition motif required for physiologic interactions is not known. We have developed a novel system using poly(ethylene glycol) (PEG) hydrogels modified with either integrin-binding peptide sequences or the selectin ligand sialyl Lewis X (SLe(X)) within a parallel plate flow chamber to examine the dynamics of leukocyte adhesion to specific ligands. The adhesive peptide sequences arginine-glycine-aspartic acid-serine (RGDS) and leucine-aspartic acid-valine (LDV) as well as sialyl Lewis X were bound to the surface of photopolymerized PEG diacrylate hydrogels. Leukocytes perfused over these gels in a parallel plate flow chamber at physiological shear rates demonstrate both rolling and firm adhesion, depending on the identity and concentration of ligand bound to the hydrogel substrate. This new system provides a unique polymer-based model for the study of interactions between leukocytes and endothelium as well as a platform to develop improved scaffolds for cardiovascular tissue engineering.

Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Publishing Authors By Initials

LJ Taite,ML Rowland,KA Ruffino,BR Smith,MB Lawrence,JL West,

For similar proteins: membrane proteins: receptors, cell surface: receptors, immunologic: integrins: receptors, leukocyte-adhesion research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, immunologic: integrins: receptors, leukocyte-adhesion research

PUBMED ID PMID:

MEDLINE DATE:

Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Annals of biomedical engineering

VOLUME: 34

Page Numbers: 1705-11

Journal Abbreviation:

ISSN: 0090-6964

DAY: 10

MONTH: 10

YEAR: 2006

Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 361512

Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Keywords Mesh Terms:

KEYWORDS: Receptors, Leukocyte-Adhesion

MESH TERMS: metabolism

Chemical & Substance for Abstract: Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Information

Substance Name: Receptors, Leukocyte-Adhesion

Registry Number: 0

Grant and Affiliation Information for Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies.

AFFILIATION: Department of Bioengineering, Rice University, Houston, TX 77005, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL070537

ACRONYM: HL

MEDLINETA: Ann Biomed Eng

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies Related Publications

• A prospective, population-based study of the role of visual impairment in motor vehicle crashes among older drivers: the SEE study.
• A quantitative, theoretical framework for understanding mammalian sleep.
• American society of transplantation symposium on B cells in transplantation: harnessing humoral immunity from rodent models to clinical practice.
• Azithromycin for control of trachoma.
• Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies.
• Causal inference for non-mortality outcomes in the presence of death.
• Changes in activity of the striatum during formation of a motor habit.
• Characterization of DNA-binding specificity and analysis of binding sites of the Pseudomonas aeruginosa global regulator, Vfr, a homologue of the Escherichia coli cAMP receptor protein.
• Defining the limits of conscientious objection in health care.
• Dopamine D2 receptor-dependent modulation of striatal NO synthase activity.
• Dynamic measures of visual function and their relationship to self-report of visual functioning.
• Endochondral bone formation from hydrogel carriers loaded with BMP2-transduced cells.
• Identifying factors which maximise the lambing performance of hoggets: a cross sectional study.
• Increased temporal and prefrontal activity in response to semantic associations in schizophrenia.
• Interaction of interleukin-6 and the BMP pathway in pulmonary smooth muscle.
• Lung cancer incidence in never smokers.
• Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents.
• Molecular effects of loss of BMPR2 signaling in smooth muscle in a transgenic mouse model of PAH.
• Morphometry of the extremely thin pulmonary blood-gas barrier in the chicken lung.
• On use of the multistage dose-response model for assessing laboratory animal carcinogenicity.
• Performance of lactating dairy cows fed whole cottonseed coated with gelatinized starch plus urea or yeast culture.
• Peritoneal dialysis: a primary care perspective.
• Poly(ethylene glycol) hydrogels conjugated with a collagenase-sensitive fluorogenic substrate to visualize collagenase activity during three-dimensional cell migration.
• Radiosensitivity, Radiogenomics & RAPPER.
• Scaling of number, size, and metabolic rate of cells with body size in mammals.
• Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2.
• The application of self-efficacy principles to audiologic rehabilitation: a tutorial.
• The prevalence of open-angle glaucoma among blacks and whites 73 years and older: the Salisbury Eye Evaluation Glaucoma Study.
• Variation in hospital admission for AMI unlikely to be attributable to smoking legislation.
• Young people who self-harm.