Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism.

Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism. Abstract Text:

Arianna Loregian,Elisa Sinigalia,Beatrice Mercorelli,Giorgio ,Donald M Coen,

The mechanisms of processivity factors of herpesvirus DNA polymerases remain poorly understood. The proposed processivity factor for human cytomegalovirus DNA polymerase is a DNA-binding protein, UL44. Previous findings, including the crystal structure of UL44, have led to the hypothesis that UL44 binds DNA as a dimer via lysine residues. To understand how UL44 interacts with DNA, we used filter-binding and electrophoretic mobility shift assays and isothermal titration calorimetry (ITC) analysis of binding to oligonucleotides. UL44 bound directly to double-stranded DNA as short as 12 bp, with apparent dissociation constants in the nanomolar range for DNAs >18 bp, suggesting a minimum DNA length for UL44 interaction. UL44 also bound single-stranded DNA, albeit with lower affinity, and for either single- or double-stranded DNA, there was no apparent sequence specificity. ITC analysis revealed that UL44 binds to duplex DNA as a dimer. Binding was endothermic, indicating an entropically driven process, likely due to release of bound ions. Consistent with this hypothesis, analysis of the relationship between binding and ionic strength indicated that, on average, 4 +/- 1 monovalent ions are released in the interaction of each monomer of UL44 with DNA. The results taken together reveal interesting implications for how UL44 may mediate processivity.

Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism. Publishing Authors By Initials

A Loregian,E Sinigalia,B Mercorelli,G ,DM Coen,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

PUBMED ID PMID:

MEDLINE DATE:

Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: 4779-91

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 7

MONTH: 07

YEAR: 2007

Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism.

AFFILIATION: Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, 35121 Padova, Italy. arianna.loregian@unipd.it

Country: England

AGENCY: United States NIAID

GRANT: AI19838

ACRONYM: AI

MEDLINETA: Nucleic Acids Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism Related Publications

• Assessment of behavioural markers of autonoetic consciousness during episodic autobiographical memory retrieval: A preliminary analysis.
• Binding parameters and thermodynamics of the interaction of the human cytomegalovirus DNA polymerase accessory protein, UL44, with DNA: implications for the processivity mechanism.
• Coherence properties of supercontinuum spectra generated in photonic crystal and tapered optical fibers.
• Detection of neonatal seizures through computerized EEG analysis.
• Diversification and co-option of RAD-like genes in the evolution of floral asymmetry.
• Electroencephalographic and neuroimaging findings in neonates undergoing extracorporeal membrane oxygenation.
• Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization.
• Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization.
• Enzymatic amplification of DNA by PCR: standard procedures and optimization.
• Enzymatic amplification of DNA by PCR: standard procedures and optimization.
• High-dose calcitriol therapy and progression of cardiac vascular calcifications.
• Hopping of a processivity factor on DNA revealed by single-molecule assays of diffusion.
• Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65.
• ICP34.5-dependent and -independent activities of salubrinal in herpes simplex virus-1 infected cells.
• In vivo fitness and virulence of a drug-resistant herpes simplex virus 1 mutant.
• Inhibition of translation by a short element in the 5' leader of the herpes simplex virus 1 DNA polymerase transcript.
• Inhibitors of the sodium potassium ATPase that impair herpes simplex virus replication identified via a chemical screening approach.
• Investigating the enhancing effect of music on autobiographical memory in mild Alzheimer's disease.
• Narcissistic temptations to cross boundaries and how to manage them.
• Performance of the CJDATS Co-Occurring Disorders Screening Instruments (CODSIs) among Minority Offenders.
• Prediction and identification of herpes simplex virus 1-encoded microRNAs.
• Prepatterning the Drosophila notum: The three genes of the iroquois complex play intrinsically distinct roles.
• Quantitation of Rare DNAs by PCR.
• Quantitation of rare DNAs by PCR.
• The Human Cytomegalovirus UL44 C Clamp Wraps around DNA.
• The Positively Charged Surface of Herpes Simplex Virus UL42 Mediates DNA Binding.
• The polymerase chain reaction.
• Vitamin D: an old prohormone with an emergent role in chronic kidney disease.
• [Histochemical research on the positivity of the benzidine-peroxide-peroxidase reaction in the thyroid gland.]
• [Not Available.]