Binding of mRNA to the bacterial translation initiation complex.

Binding of mRNA to the bacterial translation initiation complex. Research Abstract Details 

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Binding of mRNA to the bacterial translation initiation complex. Abstract Text:

Sean M Studer,Simpson Joseph,Sean M Studer,Simpson Joseph,

Translation initiation is a key step for regulating the synthesis of several proteins. In bacteria, translation initiation involves the interaction of the mRNA with the ribosomal small subunit. Additionally, translation initiation factors 1, 2, and 3, and the initiator tRNA, also assemble on the ribosomal small subunit and are essential for efficiently recruiting an mRNA for protein biosynthesis. In the following chapter, we describe fluorescence-based methods for studying the interaction of mRNA with the bacterial initiation complex. Model mRNAs with a covalently attached fluorescent probe showed an increase in fluorescence intensity when bound to the bacterial initiation complex. Utilizing the increase in fluorescence intensity upon mRNA binding to the bacterial initiation complex, we determined the equilibrium binding constants and the association and dissociation rate constants. These methods are important for quantitatively analyzing the effects of mRNA secondary structure and the role of the initiation factors in recruitment of mRNA by the bacterial initiation complex.

Binding of mRNA to the bacterial translation initiation complex. Publishing Authors By Initials

SM Studer,S Joseph,SM Studer,S Joseph,

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Binding of mRNA to the bacterial translation initiation complex. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Methods in enzymology

VOLUME: 430

Page Numbers: 31-44

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 4

MONTH: 10

YEAR: 2007

Binding of mRNA to the bacterial translation initiation complex. Information

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LANGUAGE: eng

NlmUniqueID: 212271

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Grant and Affiliation Information for Binding of mRNA to the bacterial translation initiation complex.

AFFILIATION: Department of Chemistry and Biochemistry, University of California, San Diego, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM65265

ACRONYM: GM

MEDLINETA: Methods Enzymol

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