Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells.

Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells. Research Abstract Details 

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Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells. Abstract Text:

H Sohma,H Ohkawa,T Akino,Y Kuroki,

To identify lung lamellar body (LB)-binding proteins, the fractions binding to LB-Sepharose 4B in a Ca(2+)-dependent manner from the lung soluble fractions were analyzed with Mono Q column. Four annexins (annexins III, IV, V, and VIII) were identified by partial amino acid sequence analyses as the LB-binding proteins in the lung soluble fractions. A control experiment using phospholipid (phosphatidylserine/phosphatidylglycerol/phosphtidylcholine) liposome-Sepharose 4B revealed that annexins III, IV and V were the Ca(2+)-dependent proteins binding to the column in the lung soluble fractions, while annexin VIII was not detected. Thus, annexin VIII might preferentially bind to LB. On the other hand, the only Ca(2+)-dependent LB-binding protein identified in the bronchoalveolar lavage fluids was annexin V. It was further demonstrated that annexin V was secreted by isolated alveolar type II cells from rats and that the secretion was stimulated by the addition of phorbol ester (PMA), a potent stimulator of surfactant secretion. The PMA-dependent stimulation of annexin V was attenuated by preincubation with surfactant protein-A (SP-A), a potent inhibitor of surfactant secretion. As LB is thought to be an intracellular store of pulmonary surfactant, which is secreted by alveolar type II cells, annexin V is likely to be secreted together with the lamellar body.

Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells. Publishing Authors By Initials

H Sohma,H Ohkawa,T Akino,Y Kuroki,

For similar investigative techniques: genetic techniques: sequence analysis: sequence analysis, protein research abstracts see: investigative techniques: genetic techniques: sequence analysis: sequence analysis, protein research

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Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 130

Page Numbers: 449-55

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 2001

Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells. Keywords Mesh Terms:

KEYWORDS: Sequence Analysis, Protein

MESH TERMS: metabolism

Chemical & Substance for Abstract: Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Binding of annexins to lung lamellar bodies and the PMA-stimulated secretion of annexin V from alveolar type II cells.

AFFILIATION: Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan. sohma@sapmed.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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