Binding of actinomycin D to mRNA in vivo and in vitro.

Binding of actinomycin D to mRNA in vivo and in vitro. Research Abstract Details 

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Binding of actinomycin D to mRNA in vivo and in vitro. Abstract Text:

S Toku,Y Nabeshima,K Ogata,

1. When rats received an intraperitoneal injection of [3H]actinomycin D, it bound to the RNA moiety of free and bound polysomes of rat liver. The labeling increased gradually up to 6 h or 6 + 6 h. The poly(A)-containing mRNA showed definite radioactivity and its specific activity was higher than that of rRNA, although the total radioactivity of rRNA was markedly higher than that of poly(A)-containing mRNAs. 2. An equilibrium dialysis method using rabbit globin mRNA showed that the binding constant of actinomycin D to globin mRNA was 0.056 x 10(6) M-1, and globin mRNA had 2 binding sites per mol for actinomycin D. 3. From the results of the present experiments and those described in the preceding paper, it is suggested that one of the mechanisms by which actinomycin D treatment in vivo and in vitro stimulates the template activity of mRNA may be binding to mRNA, which alters the conformation of mRNA.

Binding of actinomycin D to mRNA in vivo and in vitro. Publishing Authors By Initials

S Toku,Y Nabeshima,K Ogata,

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Binding of actinomycin D to mRNA in vivo and in vitro. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 93

Page Numbers: 361-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1983

Binding of actinomycin D to mRNA in vivo and in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Binding of actinomycin D to mRNA in vivo and in vitro. Keywords Mesh Terms:

KEYWORDS: Tritium

MESH TERMS: diagnostic use

Chemical & Substance for Abstract: Binding of actinomycin D to mRNA in vivo and in vitro. Information

Substance Name: Globins

Registry Number: 9004-22-2

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Country: JAPAN

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MEDLINETA: J Biochem

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