Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors.

Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors. Abstract Text:

Li Liu,Yun Lu,Qing Shao,Yi-Yu Cheng,Hai-Bin Qu,Li Liu,Yun Lu,Qing Shao,Yi-Yu Cheng,Hai-Bin Qu,Li Liu,Yun Lu,Qing Shao,Yi-Yu Cheng,Hai-Bin Qu,

Radix Ophiopogonis is a widely used traditional Chinese medicine. The quality of Radix Ophiopogonis available in the market varies, and some confusing or fake herbs exist. In order to improve the quality control of Radix Ophiopogonis, a novel fingerprinting method was established using HPLC coupled with UV and evaporative light-scattering detectors (ELSDs). Extraction with methanol and liquid-liquid extraction with water-saturated n-butanol were employed for the preparation of the sample solution. Chromatographic separation was performed on a Lichrospher C(18) column (250x4.6 mm id, 5.0 mum particle size) with a linear gradient elution program. UV detection at 280 nm and evaporative light-scattering detection were utilized to obtain two subfingerprinting chromatograms. A novel protocol for data processing was proposed, in order to identify and remove redundant data obtained by the two detectors, and balance the weight of the two subfingerprints on the similarity values. The method was validated and applied to quality evaluation of 16 samples of Radix Ophiopogonis and related herbs.

Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors. Publishing Authors By Initials

L Liu,Y Lu,Q Shao,YY Cheng,HB Qu,L Liu,Y Lu,Q Shao,YY Cheng,HB Qu,L Liu,Y Lu,Q Shao,YY Cheng,HB Qu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of separation science

VOLUME: 30

Page Numbers: 2628-37

Journal Abbreviation:

ISSN: 1615-9306

DAY: 17

MONTH: Nov

YEAR: 2007

Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101088554

Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors.

AFFILIATION: Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China. Fax: +86-571-88208428.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Sep Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Binary chromatographic fingerprinting for quality evaluation of Radix Ophiopogonis by high-performance liquid chromatography coupled with ultraviolet and evaporative light-scattering detectors Related Publications

• Acoustic grating fringe projector for high-speed and high-precision three-dimensional shape measurements.
• Applications of quantitative pharmacodynamic effect markers in drug target identification and therapy development.
• Designing a Signal Conditioning System with Software Calibration for Resistor-feedback Patch Clamp Amplifier.
• Optimal expression condition of recombinant RAP.
• Role of autolysin-mediated DNA release in biofilm formation of Staphylococcus epidermidis.
• Sensitive biomarker of polycyclic aromatic hydrocarbons (PAHs): urinary 1-hydroxyprene glucuronide in relation to smoking and low ambient levels of exposure.
• Systems biology approaches to metabolic and cardiovascular disorders: network perspectives of cardiovascular metabolism.
• Three-Dimensional Tracking of Single Granules in Living PC-12 Cells Employing TIRFM and WFFM.
• [Achievements in the mosquito study in China in 50 years]
• [Analysis of RPE damage after photodynamic therapy in patients with idiopathic choroidal neovascularization]
• [Analysis of the long-term outcome of anterior approach surgery on cervical spondylotic myelopathy]
• [Apoptosis of cultured cortical neurons of rat's brain induced by heroin]
• [Arthroscopically assisted anterior cruciate ligament reconstruction using patellar tendon autograft fixed with interference screw]
• [CT features and anatomic-pathologic basis of the 16-slice spiral CT for aortic dissection]
• [Coagulation behavior of Al13 species]
• [Construction of GPI-CTLA4Ig chimeric molecule and its expression on CHO-dhfr(-) cells]
• [Effect of some active Chinese herbal fraction on brain tissue proteomic profile of ischemic mouse]
• [Effects of cardiac sympathetic blockade on left ventricular diastolic function in patients with dilated cardiomyopathy and severe heart failure]
• [Electrochemical hydrodehalogenation of pentachloraphenol in aqueous solution by porous titanium loaded Pd cathode]
• [Expression of muscarinic acetylcholine receptor-11 in human retinal pigment epithelium]
• [Genetic diversity of Coptis chinensis germplasm based on ISSR analysis]
• [Impact of STAT6 signaling pathway blockade on apoptosis of human colon cancer cells]
• [Influence of chronic ethanol intake insulin sensitivity and IR, IRS-1, IRS-2 mRNA expression of liver in male rats]
• [Inhibitory effects of matrix metalloproteinase (MMP) inhibitor LY52 on expression of MMP-2 and MMP-9 and invasive ability of human ovarian carcinoma cell line SKOV3]
• [Isolation and purification of eccrine sweat glands in human skin.]
• [Medical thoracoscopy in the diagnosis of complicate pleural effusion]
• [Re-osseointegration of peri-implantitis bone defects: an experimental study in dogs]
• [Retrospective analysis on 116 cases of bradyarrhythmia treated with TCM based syndrome differentiation]
• [Studies on chemical constituents from roots of Craibiodendron henryi]
• [Study on influence between activated carbon property and immobilized biological activated carbon purification effect]