Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt.

Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt. Abstract Text:

Rui Zeng,Ying Yao,Min Han,Xiaoqin Zhao,Xiao-Cheng Liu,Juncheng Wei,Yun Luo,Juan Zhang,Jianfeng Zhou,Shixuan Wang,Ding Ma,Gang Xu,Rui Zeng,Ying Yao,Min Han,Xiaoqin Zhao,Xiao-Cheng Liu,Juncheng Wei,Yun Luo,Juan Zhang,Jianfeng Zhou,Shixuan Wang,Ding Ma,Gang Xu,

Chronic hypoxia in the renal parenchyma is thought to induce epithelial-to-mesenchymal transition (EMT), leading to fibrogenesis and ultimately end-stage renal failure. Biliverdin reductase, recently identified as a serine/threonine/tyrosine kinase that may activate phosphatidylinositol 3-kinase (PI3K) and Akt, is upregulated in response to reactive oxygen species that may accompany hypoxia. We investigated this potential role of biliverdin reductase in hypoxia-induced renal tubular EMT. Expression of biliverdin reductase was upregulated in a human proximal tubule cell line (HK-2) cultured in hypoxic conditions (1% O2), and this was accompanied by reduced expression of E-cadherin and increased expression of the mesenchymal marker vimentin. Inhibiting PI3K reversed these changes, consistent with EMT. In normoxic conditions, overexpression of biliverdin reductase promoted similar characteristics of EMT, which were also reversed by inhibiting PI3K. Furthermore, using small interfering RNA (siRNA) to knockdown biliverdin reductase, we demonstrated that the enzyme associates with phosphorylated Akt and mediates the hypoxia-induced EMT phenotype. In vivo, expression of biliverdin reductase increased in the tubular epithelia of 5/6-nephrectomized rats, and immunohistochemistry of serial sections demonstrated similar localization of phosphorylated Akt and biliverdin reductase. In conclusion, biliverdin reductase mediates hypoxia-induced EMT through a PI3K/Akt-dependent pathway.

Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt. Publishing Authors By Initials

R Zeng,Y Yao,M Han,X Zhao,XC Liu,J Wei,Y Luo,J Zhang,J Zhou,S Wang,D Ma,G Xu,R Zeng,Y Yao,M Han,X Zhao,XC Liu,J Wei,Y Luo,J Zhang,J Zhou,S Wang,D Ma,G Xu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Society of Nephrology : JA

VOLUME: 19

Page Numbers: 380-7

Journal Abbreviation: J. Am. Soc. Nephrol.

ISSN: 1533-3450

DAY: 9

MONTH: 01

YEAR: 2008

Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9013836

Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt.

AFFILIATION: Division of Nephrology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, People's Republic of China.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Soc Nephrol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt Related Publications

• Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.
• Expression of caltrin in the baculovirus system and its purification in high yield and purity by cobalt (II) affinity chromatography.
• Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat.
• [Analysis of the mutations of BRCA1 in 9 familiar breast cancer patients]
• [Application of the interaction models between the polymorphism(s) of metabolic gene(s) and environmental exposure]
• [Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-kappaB]
• [Changes in the activity of platelet L-arginine/nitric oxide pathway in hypercholesterolemia patients]
• [Clinical significance of changes in insulin resistance and secretion in multiple organ dysfunction syndrome patients complicated by trauma and hemorrhage]
• [Cloning and sequencing of the genes coding for the histidine-rich protein II of Plasmodium falciparum]
• [Contrast agents in magnetic resonance imaging: development and problems]
• [Differentially expressed genes associated with cold acclimation]
• [Effects of embryonic neural stem cells and glial cell line-derived neurotrophic factor in the repair of spinal cord injury]
• [Effects of ulinastatin on interleukin-8 during one-lung ventilation in surgery]
• [Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord]
• [Mechanism of modulation of synaptic efficacy by stress and learning]
• [Opinion on hydatidosis control by immuno-prophylaxis]
• [Oral mucosa graft combined with a local turn-over flap for urethral reconstruction in various hypospadias]
• [Pharmacognostical studies on the roots of Euphorbia prolifera]
• [Pharmacological comparison between virosecurinine and securinine]
• [Phenotyping and genotyping studies in a family with the compound heterozygosity for a deletional delta beta-thalassemia and a beta-thalassemia]
• [Properties of whole-cell potassium currents in mechanically dissociated Drosophila larval central neurons]
• [Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot]
• [Retrospective analysis of effectiveness of tonsillar squamous cell carcinoma: report of 108 cases]
• [Significance of hRad17 mRNA expression in human gastric cancer]
• [Study of functional L1 retrotransposon in human type 2 diabetes susceptibility loci]
• [Study on diarrhea disease and Escherichia coli strains harboring HPI pathogenicity island of Yersinia enterolitica in Shandong province]
• [The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice]
• [The influence of postburn administration of rhGH on the host inflammatory response]
• [The transplantation of latissimus dorsi flap of the base of T shape artery with the pedicle of the thoracodorsal artery]
• [Tissue-specific expression of Na(+)-H(+) exchanger isoforms at two developmental stages of human fetus]